Inhibition (using Hsap\KCNJ2^5XUAS.EGFP) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R27G01 and Hsap\RELA^AD.VT033301 in third instar larvae does not significantly affect short-term associative appetitive olfactory memory following training with fructose, innate odor (n-amylacetate) preference or innate fructose preference, compared to controls.

Inhibition (using Hsap\KCNJ2^5XUAS.EGFP) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R27G01 and Hsap\RELA^AD.R14E06 in third instar larvae does not significantly affect short-term associative appetitive olfactory memory following training with fructose, innate odor (n-amylacetate) preference or innate fructose preference, compared to controls; olfactory behavior is more susceptible to non-associative effects of odor-only and fructose-only exposure, compared to controls.

Inhibition (using shi^{ts1.UAS.Tag:MYC} at 31[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R27G01 and Hsap\RELA^AD.R15B01 in adults during training, consolidation or retrieval significantly reduces 2 h aversive, but not appetitive, olfactory memory, compared to controls.

Inhibition of the combined set of a subset of Scer\GAL4^GMR72B08 cells (expression restricted using Scer\GAL80^12xCRE) and neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R27G01 and Hsap\RELA^AD.R15B01 in adults during training, consolidation or retrieval, significantly reduces 2 h aversive, but not appetitive, olfactory memory, compared to controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R27G01 and Hsap\RELA^AD.R15B01 in adult males significantly reduces the amount of sleep, compared to controls; activation does not significantly affect locomotor activity of awake adult males, compared to controls.

Optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with 617 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R27G01 and Hsap\RELA^AD.R15B01 in adult females in an area of an arena does not significantly affect time spent in that area, compared to controls.

Inhibition (using shi^{ts1.IVS.p10.20xUAS}, but not shi^1.UAS, at 32[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R27G01 and Hsap\RELA^AD.R15B01 in adults during training, consolidation and testing substantially reduces aversive and attractive 2 h associative olfactory memory, compared to controls; inhibition (using shi^{ts1.IVS.p10.20xUAS} at 31-33[o]C) also significantly reduces appetitive, but not aversive, 1 min associative visual memory, compared to controls, with no effect on sugar attraction; inhibition during testing alone does not significantly reduce aversive 24 h associative olfactory memory, compared to controls; inhibition only during training and testing significantly reduces 24 h appetitive, but not 30 min aversive, associative olfactory memory towards ethanol, compared to controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa or TrpA1^{20xUAS.JFRC124} at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R27G01 and Hsap\RELA^AD.R15B01 in adults significantly reduces sleep, compared to controls, with no effect on social or locomotor behaviors.