FlyBase Allele Report: Dmel\shams[Δ34]

General Information

Symbol

Dmel\shams^Δ34

Species

D. melanogaster

Name

FlyBase ID

FBal0318496

Feature type

allele

Associated gene

Dmel\shams

Associated Insertion(s)

PBac{RBr}shams^Δ34

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

FLPase

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Lee et al., 2013)

Mutagen

FLPase

(Lee et al., 2013)

Progenitor genotype

PBac{RB}shams^e01256

(Lee et al., 2013)

PBac{RB}CG11836^e01985

(Lee et al., 2013)

Associated Insertion(s)

PBac{RBr}shams^Δ34

Caused by aberration

Df(3R)shams^Δ34

Cytology

Description

FLP/FRT-mediated recombination between the progenitor insertions resulting in deletion of 97% of the shams coding region.

(Lee et al., 2013)

Allele components

Component

Use(s)

Inserted element

PBac{RBr}

gene trap

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:25,071,710..25,074,258 [-]

Comment:

FLP/FRT-mediated recombination between the progenitor insertions resulting in a deletion of 97% of the shams coding region.

(Lee et al., 2013)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable

(Matsumoto et al., 2016, Lee et al., 2013)

viable (with Df(3R)BSC494)

(Lee et al., 2013)

Phenotype Manifest In

adult head bristle

(Matsumoto et al., 2016)

ocellar bristle | heat sensitive (with Df(3R)BSC494)

(Lee et al., 2013)

postocellar bristle | heat sensitive (with Df(3R)BSC494)

(Lee et al., 2013)

wing vein | heat sensitive (with Df(3R)BSC494)

(Lee et al., 2013)

wing vein L5

(Lee et al., 2013)

wing vein L5 (with Df(3R)BSC494)

(Lee et al., 2013)

Detailed Description

Statement

Reference

shams^Δ34 homozygotes are viable.

shams^Δ34 homozygous pharate adults display some missing bristles on the dorsal head capsule, but not on the scutellum, and eyes of normal morphology, as compared to controls.

shams^Δ34 homozygous exhibit no obvious defects in the numbers of either sensory organ precursor cells or supraalar neurons in third instar larval wing discs, as compared to controls.

(Matsumoto et al., 2016)

Homozygotes or hemizygotes survive to adulthood and exhibit a 100% loss of wing vein L5 at 25[o]C. At 30[o]C, shams^Δ34/Df(3R)BSC494 escapers exhibit partial loss of multiple wing veins, and the ocellar and postvertical bristles in the head are lost.

(Lee et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

shams^Δ34/shams[+] is a suppressor of abnormal size | dominant | adult stage | temperature conditional phenotype of N^55e11

(Pandey et al., 2018)

shams^Δ34/shams^Δ34 is a suppressor of abnormal size | dominant | adult stage | temperature conditional phenotype of N^55e11

(Pandey et al., 2018)

shams^Δ34/shams[+], Xxylt^Δcds/Xxylt^Δcds is a suppressor of abnormal size | dominant | adult stage | temperature conditional phenotype of N^55e11

(Pandey et al., 2018)

Other

Statement

Reference

O-fut1^R245AKI, shams^Δ34 has increased cell number | third instar larval stage phenotype

(Matsumoto et al., 2016)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

shams^Δ34/Df(3R)BSC494 has wing vein phenotype, non-suppressible by CG11836^+tLa

(Lee et al., 2013)

Suppressor of

Statement

Reference

shams^Δ34/shams^Δ34 is a suppressor of wing margin | temperature conditional phenotype of N^55e11

(Pandey et al., 2018)

shams^Δ34/shams^Δ34 is a suppressor of wing vein | temperature conditional phenotype of N^55e11

(Pandey et al., 2018)

shams^Δ34/shams[+], Xxylt^Δcds/Xxylt^Δcds is a suppressor of wing margin | temperature conditional phenotype of N^55e11

(Pandey et al., 2018)

shams^Δ34/shams[+], Xxylt^Δcds/Xxylt^Δcds is a suppressor of wing vein | temperature conditional phenotype of N^55e11

(Pandey et al., 2018)

shams^Δ34/shams[+] is a suppressor of wing margin | temperature conditional phenotype of N^55e11

(Pandey et al., 2018)

shams^Δ34/shams[+] is a suppressor of wing vein | temperature conditional phenotype of N^55e11

(Pandey et al., 2018)

shams^e01256/shams^Δ34 is a suppressor of wing margin phenotype of Hsap\GXYLT1^UAS.Tag:HA, Scer\GAL4^ptc.PU

(Lee et al., 2013)

shams^e01256/shams^Δ34 is a suppressor of wing margin phenotype of Hsap\XXYLT1^UAS.Tag:HA, Scer\GAL4^ptc.PU

(Lee et al., 2013)

shams^e01256/shams^Δ34 is a suppressor of wing vein L3 phenotype of Hsap\XXYLT1^UAS.Tag:HA, Scer\GAL4^ptc.PU

(Lee et al., 2013)

shams^e01256/shams^Δ34 is a suppressor of wing vein L4 phenotype of Hsap\XXYLT1^UAS.Tag:HA, Scer\GAL4^ptc.PU

(Lee et al., 2013)

Other

Statement

Reference

O-fut1^R245AKI, shams^Δ34 has wing disc | third instar larval stage phenotype

(Matsumoto et al., 2016)

O-fut1^R245AKI, shams^Δ34 has sensory mother cell | third instar larval stage phenotype

(Matsumoto et al., 2016)

O-fut1^R245AKI, shams^Δ34 has eo neuron | third instar larval stage phenotype

(Matsumoto et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The wing margin loss and wing vein thickening observed in N^55e11 hemizygous males are partially suppressed by shams^Δ34 heterozygosity and (more efficiently) homozygosity; suppression by the combination of Xxylt^Δcds homozygosity and shams^Δ34 heterozygosity is more efficient than by either single mutant.

(Pandey et al., 2018)

The third instar larval wing discs of shams^Δ34, O-fut1^R245AKI double homozygotes raised at 25[o]C of temperature exhibit increased numbers of both sensory organ precursor cells and supraalar neurons, as compared to controls.

(Matsumoto et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

shams^Δ34/Df(3R)BSC494 is rescued by shams^+tLa

(Lee et al., 2013)

shams^Δ34/Df(3R)BSC494 is rescued by shams^Tag:HA

(Lee et al., 2013)

shams^Δ34/Df(3R)BSC494 is rescued by shams^UAS.Tag:HA/Scer\GAL4^nub-AC-62

(Lee et al., 2013)

Comments

The wing vein loss phenotype of shams^Δ34/Df(3R)BSC494 animals can be rescued by shams^+tLa, shams^T:Ivir\HA1 or Scer\GAL4^nub-AC-62-mediated expression of shams^{Scer\UAS.T:Ivir\HA1}.

The loss of ocellar and postvertical bristles in the head of shams^Δ34/Df(3R)BSC494 animals is rescued by shams^+tLa.

(Lee et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

shams³⁴

(Matsumoto et al., 2016)

shams^Δ34

(Pandey et al., 2018, Lee et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

Open Close