FB2024_03 , released June 25, 2024
Allele: Dmel\Arl2Q70L.UAS
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General Information
Symbol
Dmel\Arl2Q70L.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0318770
Feature type
allele
Associated gene
Dmel\Arl2
Associated Insertion(s)
Carried in Construct
P{UAS-Arl2.Q70L}
Key Links
Transgenic product class
missense_variant
(Chen et al., 2016)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Chen et al., 2016)
Mutagen
in vitro construct
(Chen et al., 2016)
Progenitor genotype
Carried in construct
P{UAS-Arl2.Q70L}
Cytology
Description

UAS regulates expression of Arl2 full-length coding sequence containing a Q70L mutation, thus generating a constitutively active GTP-bound form of Arl2.

(Chen et al., 2016)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Arl2
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Overexpression of Arl2Q70L.Scer\UAS driven by Scer\GAL4insc.PU leads to dramatic depletion of neuroblasts in third instar larval central brains; overexpression in both type I (Scer\GAL4ase.neuro) and II (Scer\GAL4wor.PA and Scer\GAL80ase.PN) neuroblast lineages leads to neuroblast depletion.

      Overexpression of Arl2Q70L.Scer\UAS driven by Scer\GAL4insc.PU leads to more abundant microtubules in interphase neuroblasts compared to controls and around half of Arl2Q70L.Scer\UAS neuroblasts form monopolar spindles; in a microtubule regrowth assay (after ice treatment), the majority of metaphase neuroblasts show more abundant microtubules emanating from centrosomes and distance between centrosomes is increased, compared to controls. After 2min recovery after ice treatment, most mutant metaphase centrosomes contain only one large centrosome.

      (Chen et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Arl2Q70L.Scer\UAS
      Arl2Q70L.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (1)