FB2024_03 , released June 25, 2024
Allele: Dmel\spideyUAS.ORF.GW.Tag:HA
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General Information
Symbol
Dmel\spideyUAS.ORF.GW.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0319891
Feature type
allele
Associated gene
Dmel\spidey
Associated Insertion(s)
Carried in Construct
M{UAS-spidey.ORF.3xHA.GW}
Key Links
Transgenic product class
wild_type
(Bischof and FlyORF project members, 2014.6.20)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Bischof and FlyORF project members, 2014.6.20)
Mutagen
in vitro construct
(Bischof and FlyORF project members, 2014.6.20)
Progenitor genotype
Carried in construct
M{UAS-spidey.ORF.3xHA.GW}
Cytology
Description

UASt regulatory sequences drive expression of a full-length spidey open reading frame (ORF) that is tagged at the C-terminal end with three copies of the Tag:HA epitope. The ORF is flanked by a pair of incompatible FRT sites (FRT5 and FRT2), which allows for future in vivo exchange of either the promoter or the C-terminal tag sequence.

(Bischof and FlyORF project members, 2014.6.20)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
spidey
Tagged with
Tag:HA
Also carries
FRT2
FRT5
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of spideyScer\UAS.ORF.GW.T:Ivir\HA1 under the control of Scer\GAL4Desat1.PB with Scer\GAL80ts.αTub84B results in complete lethality either during prepupal stage or pharate adult stage when the flies are reared under restrictive temperature (29[o]C) and partial lethality when kept under permissive temperature (19[o]C). spideyScer\UAS.ORF.GW.T:Ivir\HA1 expressing flies also display head and spiracle eversion defects.

      Expression of spideyScer\UAS.ORF.GW.T:Ivir\HA1 under the control of Scer\GAL4Desat1.PB with Scer\GAL80ts.αTub84B leads to a significant drop in the levels of steroid hormones 20-hydroxyecdysone and makisteron A (while the amounts of their catabolic products are below detection level) in third instar larvae when the animals are reared under restrictive temperature (29[o]C) compared to controls kept under permissive temperature (19[o]C).

      (Chiang et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      spideyScer\UAS.ORF.GW.T:Ivir\HA1
      spideyUAS.ORF.GW.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (2)