Ten copies of the UAS site followed by an Hsp70 basal promoter are fused upstream of a full-length Nos cDNA. A small intron derived from the Mhc gene followed by a synthetic AT-rich 21bp sequence has been inserted between the promoter and Nos sequences. The 3' UTR from the Autographa californica nucleopolyhedrovirus (AcNPV) p10 gene is present downstream of the Nos sequences.
Expression of NosIVS.Syn21.p10.10xScer\UAS under the control of Scer\GAL4ey-OK107 does not significantly affect axon regrowth in the mushroom body gamma-lobe.
Expression of NosIVS.Syn21.p10.10xScer\UAS under the control of Scer\GAL4ey-OK107 in a NosΔ15/NosΔ15 background results in early pupal lethality.
Expression of NosIVS.Syn21.p10.10xScer\UAS under the control of Scer\GAL4GMR71G10 in NosΔall/NosΔall or NosΔN-ter/NosΔN-ter mushroom body clones leads to a dramatic delay in axon regrowth in the gamma lobe.