FB2024_03 , released June 25, 2024
Allele: Dmel\Sox21aUAS.Tag:FLAG
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General Information
Symbol
Dmel\Sox21aUAS.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0324196
Feature type
allele
Associated gene
Dmel\Sox21a
Associated Insertion(s)
Carried in Construct
P{UAS-Sox21a.T:Zzzz\FLAG}
Key Links
Transgenic product class
wild_type
(Meng and Biteau, 2015)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Meng and Biteau, 2015)
Mutagen
in vitro construct
(Meng and Biteau, 2015)
Progenitor genotype
Carried in construct
P{UAS-Sox21a.T:Zzzz\FLAG}
Cytology
Description

UAS regulatory sequences drive expression of part of the 5'UTR and the coding sequence of Sox21a. The protein is tagged at the C-terminal end with Tag:FLAG.

(Meng and Biteau, 2015)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Sox21a
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Sox21aScer\UAS.T:Zzzz\FLAG under the control of either Scer\GAL4esg-NP5130 or Scer\GAL4Dl-05151-G results in increased number of mitotic cells relative to controls.

      MARCM somatic clones in the adult gut expressing Sox21aScer\UAS.T:Zzzz\FLAG under the Scer\GAL4tub.PU driver contain significantly more cells compared to control clones but do not display any abnormalities in the ability of intestinal stem cells to differentiate (assesed by frequency of enteroendocrine cells and appearance of enterocytes in the clones).

      (Meng and Biteau, 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The increased cell proliferation observed upon the expression of Sox21aScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4esg-NP5130 is partially suppressed by co-expression of kaydsRNA.Scer\UAS when an insertion line with a strong expression is used but has no significant effect when a weak-expressing line is used.

      (Meng and Biteau, 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Sox21aScer\UAS.T:Zzzz\FLAG
      Sox21aUAS.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (1)