UAS regulatory sequences drive expression of a kinase-dead form of Hsap\PINK1 (carries the mutations K219A, D362A and D384A in the kinase domain).
The expression of Hsap\PINK1Scer\UAS.cYa under the control of Scer\GAL4Act.PU in clones within mosaic midguts results in enterocytes exhibiting a significant increase in the relative mitochondrial content during pupariation, but not at 4 or 6 hours after puparium formation, and a significant decrease in the relative amount of autophagosomes, as assessed by a Atg8a fluorescent reporter, during pupariation, as compared to non-clone enterocytes.