FB2024_04 , released June 25, 2024
Allele: Zzzz\VHHlexAop.UAS.morphotrap.int.mCherry
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General Information
Symbol
Zzzz\VHHlexAop.UAS.morphotrap.int.mCherry
Species
a. artificial
Name
FlyBase ID
FBal0326546
Feature type
allele
Associated gene
Zzzz\VHH
Associated Insertion(s)
Carried in Construct
M{lexAop-UAS-morphotrap.int.mCh}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Harmansa et al., 2017)
Progenitor genotype
Carried in construct
M{lexAop-UAS-morphotrap.int.mCh}
Cytology
Description

lexAop and UAS regulatory sequences and a minimal Hsp70 promoter (derived from the pUASTLOTattB vector) are fused upstream of a fusion protein that consists of the mCherry fluorescent tag, a transmembrane domain (Tag:M(mCd8a) and the GFP-binding VHH(GFP4) nanobody. The VHH(GFP4) nanobody is intracellular, while the mCherry tag is extracellular. The lexAop and UAS regulatory sequences are flanked by loxP and lox2272 recombinase target sites respectively (meaning that the P1cre recombinase can be used to excise one of the enhancers in a mutually exclusive manner if desired).

(Harmansa et al., 2017)
Allele components
Component
Use(s)
Regulatory region(s)
lexAop
UAS
Encoded product / tool
VHH(GFP4)
Tagged with
mCherry
Also carries
lox2272
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Zzzz\VHHlexAop.UAS.morphotrap.int.mCherry
      Zzzz\vhhGFP4Ecol\lexAop.Scer\UAS.morphotrap.int.T:Disc\RFP-mCherry
      Zzzz\vhhGFP4lexAop.UAS.morphotrap.int.mCherry
      Name Synonyms
      Secondary FlyBase IDs
        References (3)