FB2024_03 , released June 25, 2024
Allele: Dmel\Tis11RNAi.UAS.WIZ
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General Information
Symbol
Dmel\Tis11RNAi.UAS.WIZ
Species
D. melanogaster
Name
FlyBase ID
FBal0326735
Feature type
allele
Associated gene
Dmel\Tis11
Associated Insertion(s)
Carried in Construct
P{UAS-Tis11.RNAi.WIZ}
Key Links
Transgenic product class
RNAi_reagent
(McClelland et al., 2017)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(McClelland et al., 2017)
Mutagen
in vitro construct
(McClelland et al., 2017)
Progenitor genotype
Carried in construct
P{UAS-Tis11.RNAi.WIZ}
Cytology
Description

UAS regulatory sequences drive expression of an inverted repeat of a 396bp fragment of the Tis11 coding region, in tail-to-tail orientation.

(McClelland et al., 2017)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Tis11
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Intestinal stem cell MARCM clones in the adult midgut expressing Tis11dsRNA.Scer\UAS.WIZ under the control of Scer\GAL4tub.PU are significantly larger than control clones at both 3 and 7 days post clone induction, but the ratio between enteroendocrine cells and enterocytes is not perturbed. Flies expressing Tis11dsRNA.Scer\UAS.WIZ under the control of Scer\GAL4esg-NP5130 combined with tub-Gal80[ts] to limit time of expression (as well as flies of the same genotype but also carrying Scer\GAL80Su(H).GBE) display increased number of mitotic cells following a stress stimulus (either Erwinia carotovora carotovora infection or a 60 min heat-shock) but no difference is observed in the absence of a proliferative stimuli.

      No significant difference in size is observed between Tis11d00685-expressing clones (controlled by Scer\GAL4Act5C.PU) and the control ones in either larval brains or wing discs.

      (McClelland et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Tis11RNAi.UAS.WIZ
      Tis11dsRNA.Scer\UAS.WIZ
      Tis11dsRNA.UAS.WIZ
      Name Synonyms
      Secondary FlyBase IDs
        References (2)