Expression of Hsap\SETXScer\UAS.cCa under the control of either Scer\GAL4elav-C155 or Scer\GAL4Mef2.PU results in increased average number as well as area of boutons on neuromuscular junctions in third instar larvae. No defects in neural electrophysiology are observed at the junctions (no change in amplitudes of either evoked or miniature junction potential, EJP, quantal content and no significant decline in EJP amplitudes over prolonged high-frequency stimulation) with the Scer\GAL4elav-C155 driver.
Expressing Hsap\SETXWT.UAS under the control of Scer\GAL4Act5C.PU leads to larval neuromuscular junctions with significantly more and smaller boutons, as compared to controls; these boutons also show increased frequency of Futsch loops. There are no changes in neurotransmission under basal conditions (spontaneous miniature and evoked excitatory junction potential, and quantal content) or under high frequency stimulation (evoked excitatory junction potential amplitude), as compared to controls.