FlyBase Allele Report: Dmel\axed[0011]

General Information

Symbol

Dmel\axed⁰⁰¹¹

Species

D. melanogaster

Name

FlyBase ID

FBal0327537

Feature type

allele

Associated gene

Dmel\axed

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Neukomm et al., 2017)

Progenitor genotype

Cytology

Description

16bp deletion in exon 3 of axed.

(Neukomm et al., 2017)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3L:6,616,705..6,616,720 [+]

Comment:

16bp deletion in the second coding exon of axed.

(Neukomm et al., 2017)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal wound healing | adult stage | somatic clone

(Neukomm et al., 2017)

lethal | recessive

(Neukomm et al., 2017)

Phenotype Manifest In

axon | adult stage | somatic clone

(Neukomm et al., 2017)

Detailed Description

Statement

Reference

Flies carrying axed⁰⁰¹¹ mutant somatic clones show defective injury-induced axon degeneration: severed axons (either in sensory neurons in the wing or in cholinergic olfactory receptor neurons) remain intact for a full week after an axotomy (or even 50 days in the latter case), instead of being cleared away. axed⁰⁰¹¹ MARCM clones of gamma mushroom body neurons do not show any defects in developmental axon or dendrite pruning.

(Neukomm et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Nmnat^Δ4790-1, axed⁰⁰¹¹ has abnormal wound healing | adult stage phenotype, non-suppressible by Scer\GAL4^VGlut-OK371/Sarm^{ΔARM.UAS.Tag:MYC}

(Neukomm et al., 2017)

Suppressor of

Statement

Reference

axed⁰⁰¹¹ is a suppressor of abnormal neuroanatomy | somatic clone | adult stage phenotype of Nmnat^GD8082, Scer\GAL4^VGlut-OK371

(Neukomm et al., 2017)

axed⁰⁰¹¹ is a suppressor of abnormal neuroanatomy | somatic clone | adult stage phenotype of Nmnat^Δ4790-1

(Neukomm et al., 2017)

axed⁰⁰¹¹ is a suppressor of abnormal neuroanatomy | somatic clone | adult stage phenotype of Sarm^{ΔARM.UAS.Tag:MYC}, Scer\GAL4^VGlut-OK371

(Neukomm et al., 2017)

Other

Statement

Reference

Nmnat^Δ4790-1, axed⁰⁰¹¹ has abnormal stress response | somatic clone | adult stage phenotype

(Hsu et al., 2021)

Nmnat^Δ4790-1, axed⁰⁰¹¹ has abnormal wound healing | adult stage phenotype

(Neukomm et al., 2017)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Nmnat^Δ4790-1, axed⁰⁰¹¹ has sensory neuron | somatic clone | adult stage phenotype, non-suppressible by Scer\GAL4^VGlut-OK371/Sarm^{ΔARM.UAS.Tag:MYC}

(Neukomm et al., 2017)

Nmnat^Δ4790-1, axed⁰⁰¹¹ has axon | somatic clone | adult stage phenotype, non-suppressible by Scer\GAL4^VGlut-OK371/Sarm^{ΔARM.UAS.Tag:MYC}

(Neukomm et al., 2017)

Nmnat^Δ4790-1, axed⁰⁰¹¹ has neuronal cell body | somatic clone | adult stage phenotype, non-suppressible by Scer\GAL4^VGlut-OK371/Sarm^{ΔARM.UAS.Tag:MYC}

(Neukomm et al., 2017)

Suppressor of

Statement

Reference

axed⁰⁰¹¹ is a suppressor of sensory neuron | somatic clone | adult stage phenotype of Nmnat^Δ4790-1

(Neukomm et al., 2017)

axed⁰⁰¹¹ is a suppressor of axon | somatic clone | adult stage phenotype of Nmnat^Δ4790-1

(Neukomm et al., 2017)

axed⁰⁰¹¹ is a suppressor of neuronal cell body | somatic clone | adult stage phenotype of Nmnat^Δ4790-1

(Neukomm et al., 2017)

axed⁰⁰¹¹ is a suppressor of sensory neuron | somatic clone | adult stage phenotype of Sarm^{ΔARM.UAS.Tag:MYC}, Scer\GAL4^VGlut-OK371

(Neukomm et al., 2017)

axed⁰⁰¹¹ is a suppressor of axon | somatic clone | adult stage phenotype of Sarm^{ΔARM.UAS.Tag:MYC}, Scer\GAL4^VGlut-OK371

(Neukomm et al., 2017)

axed⁰⁰¹¹ is a suppressor of sensory neuron | somatic clone | adult stage phenotype of Nmnat^GD8082, Scer\GAL4^VGlut-OK371

(Neukomm et al., 2017)

axed⁰⁰¹¹ is a suppressor of axon | somatic clone | adult stage phenotype of Nmnat^GD8082, Scer\GAL4^VGlut-OK371

(Neukomm et al., 2017)

axed⁰⁰¹¹ is a suppressor of neuronal cell body | somatic clone | adult stage phenotype of Nmnat^GD8082, Scer\GAL4^VGlut-OK371

(Neukomm et al., 2017)

Other

Statement

Reference

Nmnat^Δ4790-1, axed⁰⁰¹¹ has axon | somatic clone | adult stage phenotype

(Hsu et al., 2021)

Nmnat^Δ4790-1, axed⁰⁰¹¹ has wing nerve | somatic clone phenotype

(Hsu et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

MARCM clones in sensory neurons in the adult wing expressing Ect4^{ΔARM.Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^VGlut-OK371 undergo spontaneous cell body and axon degeneration which can be suppressed by combination with axed⁰⁰¹¹.

The axon and cell body degeneration in sensory neuron clones in the adult wing mutant for Nmnat^Δ4790-1 or expressing Nmnat^GD8082 under the control of Scer\GAL4^VGlut-OK371 can be fully rescued by combination with axed⁰⁰¹¹.

The Nmnat^Δ4790-1,axed⁰⁰¹¹ double mutant clones display defective injury-induced axon degeneration (severed axons remain intact following an axotomy, instead of being cleared away) which cannot be overcome by expressing Ect4^{ΔARM.Scer\UAS.T:Hsap\MYC} under the Scer\GAL4^VGlut-OK371 driver in the mutant clones.

(Neukomm et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

axed²⁰⁹⁴

(Neukomm et al., 2017)

axed^MI13270

(Neukomm et al., 2017)

Rescued by

axed⁰⁰¹¹ is rescued by Scer\GAL4^VGlut-OK371/axed^UAS.long

(Neukomm et al., 2017)

Partially rescued by

axed⁰⁰¹¹ is partially rescued by Scer\GAL4^VGlut-OK371/axed^ΔBTB.UAS

(Neukomm et al., 2017)

axed⁰⁰¹¹ is partially rescued by axed^ΔBACK.UAS/Scer\GAL4^VGlut-OK371

(Neukomm et al., 2017)

Not rescued by

axed⁰⁰¹¹ is not rescued by axed^ΔCterm.UAS/Scer\GAL4^VGlut-OK371

(Neukomm et al., 2017)

Comments

Any of the following: axed⁰⁰¹¹, axed^CrisprA, axed^CrisprB, axed^CrisprC, axed^Ex07, axed^Ex65 or axed^Ex96 fails to complement the lethality of axed⁰⁰¹¹ mutants, while Ect4⁸⁹⁶ fails to do so.

The defective injury-induced axon degeneration observed in flies carrying axed⁰⁰¹¹ mutant neural clones (severed axons in sensory neurons in the adult wing remain intact following an axotomy, instead of being cleared away) can be rescued by clonal Scer\GAL4^VGlut-OK371-driven expression of any of the following: axed^{Scer\UAS.long}, axed^{ΔBTB.Scer\UAS}, axed^{ΔBACK.Scer\UAS}, axed^{ΔCterm.Scer\UAS}.

(Neukomm et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

3L.0011

(Neukomm et al., 2017)

axed⁰⁰¹¹

(Hsu et al., 2021, Farley et al., 2018, Neukomm et al., 2017)

axed^3L11

(Hsu et al., 2021)

Name Synonyms

Secondary FlyBase IDs

References (3)

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