FlyBase Allele Report: Dmel\CTCF[P30.6]

General Information

Symbol

Dmel\CTCF^P30.6

Species

D. melanogaster

Name

FlyBase ID

FBal0336941

Feature type

allele

Associated gene

Dmel\CTCF

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Mohan et al., 2007)

Progenitor genotype

CTCF^GE22007

(Mohan et al., 2007)

Cytology

Description

Imprecise excision of P{EP}CTCF^GE22007 generates a deletion in CTCF from nucleotide -537 upstream of the transcriptional start site to position +15.

(Mohan et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3L:7,356,681..7,357,233 [-]

Comment:

Deletion resulting from the imprecise excsion of P{EP}CTCF^GE22007 generates a deletion in CTCF from nucleotide -537 upstream of the transcriptional start site to position +15 (reported relative to previously annotated transcription start site at 3L:7356696 , release 6 coordinate).

(Mohan et al., 2007)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdomen | adult stage (with CTCF^KO)

(Gambetta and Furlong, 2018)

abdomen | adult stage (with CTCF^y+1)

(Gambetta and Furlong, 2018)

abdominal segment 5

(Mohan et al., 2007)

abdominal segment 5 (with Df(3L)0463)

(Mohan et al., 2007)

abdominal segment | adult stage (with CTCF^KO)

(Gambetta and Furlong, 2018)

abdominal segment | adult stage (with CTCF^y+1)

(Gambetta and Furlong, 2018)

abdominal sternite 5

(Mohan et al., 2007)

abdominal sternite 5 (with Df(3L)0463)

(Mohan et al., 2007)

abdominal sternite 6 & abdominal sternite bristle

(Mohan et al., 2007)

abdominal sternite 6 & abdominal sternite bristle (with Df(3L)0463)

(Mohan et al., 2007)

abdominal tergite 4

(Mohan et al., 2007)

abdominal tergite 4 (with Df(3L)0463)

(Mohan et al., 2007)

abdominal tergite 5

(Mohan et al., 2007)

abdominal tergite 5 (with Df(3L)0463)

(Mohan et al., 2007)

abdominal tergite 7

(Mohan et al., 2007)

abdominal tergite 7 (with Df(3L)0463)

(Mohan et al., 2007)

abdominal tergite 7 & abdominal sternite bristle

(Mohan et al., 2007)

male genitalia

(Mohan et al., 2007)

male genitalia (with Df(3L)0463)

(Mohan et al., 2007)

Detailed Description

Statement

Reference

CTCF^KO/CTCF^P30.6 and CTCF^P30.6/CTCF^y+1 transheterozygous zygotic mutants mostly reach adulthood, with some dying as pharate adults.

CTCF^KO/CTCF^P30.6 and CTCF^y+1/CTCF^P30.6 zygotic mutant adults exhibit homeotic transformations in the abdomen.

(Gambetta and Furlong, 2018)

CTCF^P30.6 homozygotes and CTCF^P30.6/Df(3L)0463 are pharate lethal. Male genitalia are rotated by 40[o]-90[o] in 100% of cases. Males exhibit A4 tergites with patchy pigmentation. A5 tergites show a few white spots, the A5 sternite is elongated into a banana shape. Eight bristles are found on the sixth sternite, and the seventh tergite is smaller than in wild-type. CTCF^P30.6 homozygous females exhibit A7 sternites with up to 11 bristles pointing vertically downwards.

(Mohan et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

CTCF^P30.6/Df(3L)0463 is a suppressor of visible phenotype of Fab-8^60.85.1

(Mohan et al., 2007)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

A low but significant relief in Fab-8^60.85.1 enhancer blocking is found in a CTCF^P30.6/Df(3L)0463 background. All trans-heterozygote females show an increase in eye pigmentation compared with nearly all heterozygotes.

(Mohan et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

CTCF^P30.6 is rescued by CTCF^+t5084

(Mohan et al., 2007)

CTCF^P30.6/Df(3L)0463 is rescued by CTCF^+t5084

(Mohan et al., 2007)

Comments

The majority of CTCF^P30.6 flies are fully rescued by the presence of CTCF^+t.5084, with a few showing various degrees of held-out wings and are unable to walk properly. The rescued flies are fertile and can be used to establish a breeding stock.

The majority of CTCF^P30.6/Df(3L)0463 flies are fully rescued by the presence of CTCF^+t.5084, with a few showing various degrees of held-out wings and are unable to walk properly. The rescued flies are fertile and can be used to establish a breeding stock.

(Mohan et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

CTCF^30.6

(Gambetta and Furlong, 2018)

CTCF^P30.6

(Mohan et al., 2007)

CTCF^p30.6

(Mohan et al., 2007)

dCTCF^30.6

(Bonchuk et al., 2015)

dCTCF^p30.6

(Bartkuhn et al., 2009)

Name Synonyms

Secondary FlyBase IDs

FBal0241198
FBal0221168

References (4)

Report Sections

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