FB2024_02 , released April 23, 2024
Allele: Dmel\sqhEE.UAS.ALD.mCherry
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General Information
Symbol
Dmel\sqhEE.UAS.ALD.mCherry
Species
D. melanogaster
Name
FlyBase ID
FBal0337946
Feature type
allele
Associated gene
Dmel\sqh
Associated Insertion(s)
Carried in Construct
PBac{UAS-ALD-sqh.EE.mCh}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct - regulatory fusion
(Tsankova et al., 2017)
in vitro construct - coding region fusion
(Tsankova et al., 2017)
Progenitor genotype
Carried in construct
PBac{UAS-ALD-sqh.EE.mCh}
Cytology
Description

UAS regulatory sequences drives expression of a constitutively active form of sqh. The protein is tagged at the N-terminal end with the Tag:ALD(insc) apical localization domain and at the C-terminal end with mCherry.

(Tsankova et al., 2017)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
sqh
Tagged with
mCherry
Tag:ALD(insc)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of sqhEE.Scer\UAS.ALD.T:Disc\RFP-mCherry under the control of Scer\GAL4wor.PA (thus forcing constitutively activated Myosin to the apical cortex) in third instar larval neuroblasts leads to abnormalities in cell shape changes from early anaphase to telophase during asymmetric cell division: the furrowing is initiated closer to the apical surface than in wild-type neuroblast (and no subsequent basal shifting of the ingression is observed). Occasionally, the sqhEE.Scer\UAS.ALD.T:Disc\RFP-mCherry expression can cause the neuroblasts to divide symmetrically or with inverted physical asymmetry.

      (Tsankova et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      sqhEE.Scer\UAS.ALD.T:Disc\RFP-mCherry
      sqhEE.UAS.ALD.mCherry
      Name Synonyms
      Secondary FlyBase IDs
        References (1)