Deletion extending from -787 to +468 relative to the sphe start site (revision of nucleotide level data in FBrf0237463).
Imprecise excision of Mi{ET1}spheMB11555, resulting in a deletion that affects sphe. At the protein level, the first 74 amino acids residues of sphe are missing, which include the signal peptide and 50 amino acids residues of the catalytic domain, including the His residue from the catalytic triad.
Deletion resulting from the imprecise excision of Mi{ET1}spheMB11555 that starts at the insertion site and includes 835 bp of upstream sequence as well as the first exon of sphe. The junction sequences are ATTACCTTG/AAACCTGT (upstream) and CCATTATAT/ACAATA (downstream).
spheΔ104 mutants show significantly increased mortality upon infection with the Gram-positive bacterium Staphylococcus aureus or with the Gram positive bacterium Enterococcus faecalis, but not with the protease-deficient Enterococcus faecalis strain TX5128, the Gram-positive bacterium Enterococcus faecium or the entomopathogenic fungus Beauveria bassiana, as compared to controls.
spheΔ104 is rescued by Scer\GAL4Act5C.PU/spheUAS.Tag:MYC
