FB2024_03 , released June 25, 2024
Allele: Scer\GAL4NP0534
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General Information
Symbol
Scer\GAL4NP0534
Species
S. cerevisiae
Name
FlyBase ID
FBal0340832
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Genomic Maps

Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Progenitor genotype
    Associated Insertion(s)
    Cytology
    Description
    Allele components
    Component
    Use(s)
    Inserted element
    Mutations Mapped to the Genome
    Curation Data
    Variant Molecular Consequences
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
    Expression Data
    Reporter Expression
    distribution deduced from reporter (Gal4 UAS)
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
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    Modifiers Based on Experimental Evidence ( 0 )
    Disease
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    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease-implicated variant(s)
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    Inhibition (using Hsap\KCNJ2UAS.EGFP) of the combined set of the Scer\FLP1276B subset (with excised Scer\GAL80αTub84B.PG) of Scer\GAL40098-G4 cells and the Scer\GAL4NP0534 cells, in combination with a Gal80[ts] transgene to restrict expression to the adult stage, in females significantly increases proboscis extension in response to bitter solution and significantly increases bitter solution consumption, compared to controls, but overconsumption is significantly reduced compared to inactivation of Scer\FLP1276B-expressing Scer\GAL40098-G4 cells alone.

    Inhibition (using Ctet\TeTxLCTNT.UAS) of Scer\GAL4NP0534 cells in adult females significantly reduces ingestion rate and volume per pump during fluid ingestion, with no effect on pump frequency, compared to controls; cibarial filling and emptying times are not greatly altered, compared to controls, but significantly more fluid is pumped back to the proboscis, rather than to the esophagus.

    Thermogenetic activation (using TrpA1UAS.(B).cKa at 32[o]C) of a subset of Scer\GAL4NP0534 cells (restricted using Scer\GAL80tsh-GAL80) in adult females does not cause any obvious cibarial pumping phenotype, compared to controls.

    External Data
    Interactions
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    Phenotypic Class
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    Additional Comments
    Genetic Interactions
    Statement
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    Xenogenetic Interactions
    Statement
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    Complementation and Rescue Data
    Comments
    Images (0)
    Mutant
    Wild-type
    Stocks (1)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (1)
    Reported As
    Symbol Synonym
    Scer\GAL4NP0534
    Name Synonyms
    Secondary FlyBase IDs
      References (2)