FB2024_02 , released April 23, 2024
Allele: Dmel\Atx2KI.ΔcIDR-GFP
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General Information
Symbol
Dmel\Atx2KI.ΔcIDR-GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0343437
Feature type
allele
Associated gene
Dmel\Atx2
Associated Insertion(s)
TI{TI}Atx2KI.ΔcIDR-GFP
Carried in Construct
Also Known As
Atx2-ΔcIDR-GFP
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Bakthavachalu et al., 2018)
gene targeting by homologous recombination
(Bakthavachalu et al., 2018)
Progenitor genotype
Associated Insertion(s)
TI{TI}Atx2KI.ΔcIDR-GFP
Cytology
Description

Sequence encoding the C-terminal intrinsically disordered region (IDR) has been deleted from the endogenous Atx2 locus. A GFP tag has been introduced at the C-terminal end of the open reading frame. In addition, a Disc\RFPDsRed.3xP3.cUa marker allele has been inserted into the first intron, transcribed in the opposite orientation to Atx2, as part of the targeting event.

(Bakthavachalu et al., 2018)
Allele components
Component
Use(s)
Tagged with
GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  Huntington's disease
      modeled by Hsap\HTTQ138.UAS.mRFP1
      (Huelsmeier et al., 2021)
      modeled by Hsap\HTTQ97.ex1.UAS
      (Huelsmeier et al., 2021)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Atx2KI.ΔcIDR-GFP significantly decreases long-term olfactory habituation, but not short-term olfactory habituation, as compared to controls.

      (Bakthavachalu et al., 2018)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      At 22[o]C and 25[o]C, expressing Zzzz\poly-GR50.UAS.EGFP under the control of Scer\GAL4GMR.PF induces pupal lethality or visibly necrotic eyes in a small proportion of Atx2KI.ΔcIDR-GFP homozygotes; these phenotypes are not observed at 18[o]C. No defects are induced by Zzzz\poly-GA50.UAS.EGFP expression.

      Expressing Hsap\FUSUAS.cBa.Tag:HA under the control of Scer\GAL4GMR.PF induces mild-to-severe eye defects in almost half of Atx2KI.ΔcIDR-GFP homozygotes.

      (Bakthavachalu et al., 2018)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Atx2KI.ΔcIDR-GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (2)