FB2024_03 , released June 25, 2024
Allele: Dmel\bchs17M
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General Information
Symbol
Dmel\bchs17M
Species
D. melanogaster
Name
FlyBase ID
FBal0344942
Feature type
allele
Associated gene
Dmel\bchs
Associated Insertion(s)
Carried in Construct
Key Links
Allele class

hypomorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

hypomorphic allele - genetic evidence

(Sim et al., 2019)
Mutagen
P-element activity
(Sim et al., 2019)
Progenitor genotype
bchs17
(Sim et al., 2019, Hebbar et al., 2015)
Cytology
Description

Precise excision of the P{EP}EP2299 insertion present on the progenitor bchs17 chromosome. The W2640term lesion within the bchs coding sequence is still present; this is expected to disrupt isoforms 1 and 2.

(Sim et al., 2019)

The P{EP}EP2299 insertion that is present on the progenitor bchs17 chromosome has been precisely excised.

(Hebbar et al., 2015)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      bchs17M/Df(2L)cl7 transheterozygous larvae exhibit a mild decrease in the number of RP2 motor neurons, compared to controls. Their brains exhibit decreased autophagosome number (Atg8 spots/cell) and increased autophagic flux.

      Cultured larval neurons exhibit significantly more Atg5-positive vesicles, particularly enriched in the neurites, and significantly fewer Atg8-positive autophagosomes, as compared to controls. Additional expression of bchsUAS.1.GFP under the control of Scer\GAL4eve.PU fully suppresses the fewer autophagosomes and even leads to a severe increase in their number. Both Atg5-positive vesicles and Atg8-positive autophagosomes accumulate in the neurites in differentiated (i.e. 4-day cultured) neurons.

      (Sim et al., 2019)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhanced by
      Statement
      Reference

      bchs17M/Df(2L)cl7 has decreased cell number | larval stage phenotype, non-enhanceable by Atg7[+]/Atg7d77

      (Sim et al., 2019)
      Suppressed by
      NOT suppressed by
      Statement
      Reference

      bchs17M/Df(2L)cl7 has decreased cell number | larval stage phenotype, non-suppressible by Atg7[+]/Atg7d77

      (Sim et al., 2019)
      Phenotype Manifest In
      NOT Enhanced by
      Statement
      Reference

      bchs17M/Df(2L)cl7 has larval RP2 motor neuron | larval stage phenotype, non-enhanceable by Atg7[+]/Atg7d77

      (Sim et al., 2019)
      Suppressed by
      NOT suppressed by
      Statement
      Reference

      bchs17M/Df(2L)cl7 has larval RP2 motor neuron | larval stage phenotype, non-suppressible by Atg7[+]/Atg7d77

      (Sim et al., 2019)
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescued by

      bchs17M/Df(2L)cl7 is partially rescued by Scer\GAL4eve.PU/bchsUAS.1.GFP

      (Sim et al., 2019)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      bchs17(M)
      (Sim et al., 2019)
      bchs17M
      (Hebbar et al., 2015)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)