FB2024_03 , released June 25, 2024
Allele: Dmel\Zdhhc8KO1
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General Information
Symbol
Dmel\Zdhhc8KO1
Species
D. melanogaster
Name
FlyBase ID
FBal0346169
Feature type
allele
Associated gene
Dmel\Zdhhc8
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
gene targeting by homologous recombination
(Strassburger et al., 2019)
Progenitor genotype
Mi{MIC}Zdhhc8MI01245
(Strassburger et al., 2019)
Caused by aberration
Df(1)Zdhhc8KO1
Cytology
Description

The MIMIC cassette of the progenitor is replaced by Recombination Mediated Cassette Exchange (RMCE) with a knockout cassette, removing the majority of Zdhhc8 as well as the nested gene CG34450.

(Strassburger et al., 2019)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Zdhhc8KO1 homozygous larvae show molting defects: many fail to molt and remain L2 for a few days, continuing to grow to a size larger than control L2 larvae; other individuals molt to L3, but fail to grow and do not reach the size of control L3 larvae; occasionally, dead L3 larvae have their L2 carcass still attached to the mouth hooks; late L3 larvae weight less than control larvae. No tissue overgrowth is observed.

      Zdhhc8KO1 homozygous clones do not present any obvious defects in cell polarity, as cells are morphologically normal, are in a monolayer and present no defects in the sub-cellular localization of Dlg protein, as compared to neighboring cells.

      (Strassburger et al., 2019)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Other
      Statement
      Reference

      Zdhhc8[+]/Zdhhc8KO1, scribunspecified has viable phenotype

      (Strassburger et al., 2019)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Zdhhc8KO1, scribunspecified double heterozygotes are viable.

      (Strassburger et al., 2019)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Zdhhc8KO1
      dZDHHC8KO1
      (Strassburger et al., 2019)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)