FB2024_03 , released June 25, 2024
Allele: Dmel\HWA.UAS
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General Information
Symbol
Dmel\HWA.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0347588
Feature type
allele
Associated gene
Dmel\H
Associated Insertion(s)
Carried in Construct
M{UAS-H.WA}
Key Links
Transgenic product class
missense_variant
(Smylla et al., 2019)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Smylla et al., 2019)
Mutagen
in vitro construct
(Smylla et al., 2019)
Progenitor genotype
Carried in construct
M{UAS-H.WA}
Cytology
Description

UASt regulatory sequences drive expression of H that has been mutated to carry the amino acid replacement W258A.

(Smylla et al., 2019)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
H
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expressing HWA.UAS under the control of Scer\GAL4GMR.PU results in slightly smaller eyes with a rough appearance, fused or collapsed ommatidia and ectopic or absent interommatidial bristles.

      Expressing HWA.UAS under the control of Scer\GAL4sd.PU leads to overall reduced wing area, vein thickening and ectopic nets of veins at the distal part of the longitudinal veins L2 and L5 when flies are raised at 18[o]C. At a rearing temperature of 25[o]C, wings display a further area reduction, incisions of the wing margin at the tip and ectopic bristles along ectopic veins. Under the control of Scer\GAL4bi.PU, wings are significantly smaller, with incisions of the wing margin at the tip and ectopic veins at the distal part of L2 and L5 (but no ectopic bristles) at 18[o]C, and more severely at 25[o]C.

      (Smylla et al., 2019)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      HWA.UAS, Scer\GAL4sd.PU has wing phenotype, enhanceable by Su(H)UAS.cMa, Scer\GAL4sd.PU

      (Smylla et al., 2019)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expressing Su(H)UAS.cMa with HWA.UAS under the control of Scer\GAL4sd.PU results in rudimentary wings and ectopic bristle tufts mainly on the scutellum when flies are raised at 18[o]C, and complete wing loss with more scutellar bristles at 25[o]C. Adults are barely viable and need assistance at eclosion from the pupal case.

      (Smylla et al., 2019)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      HWA.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (1)