FB2024_04 , released June 25, 2024
Allele: Scer\FLP1Gad1-2A-FLP
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General Information
Symbol
Scer\FLP1Gad1-2A-FLP
Species
S. cerevisiae
Name
FlyBase ID
FBal0357304
Feature type
allele
Associated gene
Scer\FLP1
Associated Insertion(s)
TI{2A-FLP}Gad12A-FLP
Carried in Construct
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
phiC31 integrase
(Deng et al., 2019)
cre recombinase
(Deng et al., 2019)
Progenitor genotype
TI{TI}Gad1attP
(Deng et al., 2019)
Associated Insertion(s)
TI{2A-FLP}Gad12A-FLP
Cytology
Description

A sequence cassette has been introduced into the attP site present in TI{TI}Gad1attP, restoring the deleted Gad1 sequence and tagging it with 2xTag:MYC. The tag is followed by a 2A-FLP cassette, resulting in the FLP recombinase being expressed as a separate protein under the control of the endogenous Gad1 regulatory sequences. A single loxP site is present downstream of the recombinase sequence (markers present in the progenitor insertion and donor plasmid have been removed using P1cre).

(Deng et al., 2019)
Allele components
Component
Use(s)
Inserted element
TI{2A-FLP}
Encoded product / tool
FLP
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\FLP1Gad1-2A-FLP
      Name Synonyms
      Secondary FlyBase IDs
        References (1)