FlyBase Allele Report: Dmel\Usp14[Δ32]

General Information

Symbol

Dmel\Usp14^Δ32

Species

D. melanogaster

Name

FlyBase ID

FBal0358639

Feature type

allele

Associated gene

Dmel\Usp14

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

Nature of the Allele

Allele class

loss of function allele

(Kovács et al., 2020)

Mutagen

CRISPR/Cas9

(Kovács et al., 2020)

gene targeting by homologous recombination

(Kovács et al., 2020)

Progenitor genotype

Cytology

Description

A single base pair insertion 3bp downstream of the start codon results in a frameshift and premature stop codon 16bp after the transcription start.

(Kovács et al., 2020)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

insertion

2L:10,314,305..10,314,306 [-]

Inserted_sequence:

Comment:

The insertion of a C nucleotide after the second codon of Usp14 generates a frameshift and a premature stop codon 16 base pairs downstream of the translation start site.

(Kovács et al., 2020)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

male sterile

(Kovács et al., 2020)

male sterile (with Df(2L)BSC143)

(Kovács et al., 2020)

viable

(Kovács et al., 2020)

Phenotype Manifest In

elongation stage spermatid

(Kovács et al., 2020)

sperm individualization complex

(Kovács et al., 2020)

testis

(Kovács et al., 2020)

Detailed Description

Statement

Reference

Usp14^Δ32 testes exhibit sperm individualization defects, the majority of individualization complexes lacks synchronous movements of actin cones and a high proportion of individual actin cones spread along the sperm bundles when compared to controls while they have elongated spermatids similar to controls.

(Kovács et al., 2020)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Ubi-p63E^EY07341, Usp14^Δ32 has abnormal meiotic cell cycle | male | adult stage phenotype

(Kovács et al., 2020)

Phenotype Manifest In

Enhanced by

Statement

Reference

Usp14^Δ32 has testis phenotype, enhanceable by Ubi-p63E^EY07341

(Kovács et al., 2020)

Other

Statement

Reference

Ubi-p63E^EY07341, Usp14^Δ32 has spermatocyte phenotype

(Kovács et al., 2020)

Ubi-p63E^EY07341, Usp14^Δ32 has elongation stage spermatid phenotype

(Kovács et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

Spermatocytes from the Usp14^Δ32 Ubi-p63E^EY07341 double mutants have arrested meiosis and do not exhibit any postmeiotic or elongated spermatocytes when compared to controls.

(Kovács et al., 2020)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Usp14^Δ32 is rescued by Usp14^UAS.cKa/Scer\GAL4^VP16.bam

(Kovács et al., 2020)

Not rescued by

Usp14^Δ32 is not rescued by Scer\GAL4^Act5C.PU/Usp14^UAS.cKa

(Kovács et al., 2020)

Comments