UASt regulates expression of the full-length 2N4R form of Hsap\MAPT in which 14 Ser/Thr sites (Thr111, Thr153, Ser175, Thr181, Ser199, Ser202, Thr205, Thr212, Thr217, Thr231, Ser235, Ser396, Ser404 and Ser422) are changed to alanines mimicking non-phosphorylation. Phosphorylation status affects pathogenicity in human patients.
Expressing Hsap\MAPTAPFL.UAS under the control of Scer\GAL4GMR.PF leads to a rough eye phenotype on the posterior part of the eye, with disruption of the photoreceptor organization. Expression under the control of Scer\GAL4Gad1.3.098 leads to decreased locomotion (decreased walking distance, speed and increased wobbling).