UASt regulatory sequences drive expression of the Fmr1 coding region (amplified from the LD09557 cDNA), carrying a I307N substitution in the KH2 domain. This change is equivalent to a I304N change in the orthologous human FMR1 gene, a variant associated with Fragile X Syndrome. The coding sequence is tagged at the N-terminal end with EGFP.
T10105109A
I307N | Fmr1-PA; I307N | Fmr1-PB; I307N | Fmr1-PC; I307N | Fmr1-PD; I269N | Fmr1-PE; I269N | Fmr1-PF; I355N | Fmr1-PG; I355N | Fmr1-PH; I269N | Fmr1-PI; I307N | Fmr1-PJ; I307N | Fmr1-PK
I307N
Analogous mutation in human FMR1 implicated in Fragile X Syndrome; mutation carried on in vitro construct; site of nucleotide substitution in fly gene inferred by FlyBase curator based on reported amino acid change.