FB2024_03 , released June 25, 2024
Gene: Dmel\mus201
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General Information
Symbol
Dmel\mus201
Species
D. melanogaster
Name
mutagen-sensitive 201
Annotation Symbol
CG10890
Feature Type
protein_coding_gene
FlyBase ID
FBgn0002887
Gene Model Status
Current
Stock Availability
12 publicly available
Gene Summary
mutagen-sensitive 201 (mus201) encodes a protein involved in UV-damage excision repair. [Date last reviewed: 2019-09-19] (FlyBase Gene Snapshot)
All Summaries
Also Known As

mus(2)201, mus-201, XPG, ESTS:55A11T , DmXPG

Key Links
Genomic Location
Cytogenetic map
29C4-29C5
Sequence location
Recombination map
2-32
RefSeq locus
NT_033779 REGION:8438169..8442342
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (9 terms)
Molecular Function (5 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
enables 5'-flap endonuclease activity
inferred from biological aspect of ancestor with PANTHER:PTN000118612
(GO Reference Genome Project, 2011-)
enables DNA binding
inferred from electronic annotation with InterPro:IPR008918
(InterPro Project Members, 2004-)
enables endonuclease activity
inferred from electronic annotation with InterPro:IPR001044
(InterPro Project Members, 2004-)
enables nuclease activity
inferred from electronic annotation with InterPro:IPR006086
(InterPro Project Members, 2004-)
inferred from electronic annotation with InterPro:IPR006085, InterPro:IPR006086
(InterPro Project Members, 2004-)
enables single-stranded DNA binding
inferred from electronic annotation with InterPro:IPR001044
(InterPro Project Members, 2004-)
Biological Process (3 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
involved_in UV-damage excision repair
inferred from mutant phenotype
(Negishi et al., 2001)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in double-strand break repair via homologous recombination
inferred from biological aspect of ancestor with PANTHER:PTN004229235
(GO Reference Genome Project, 2011-)
involved_in nucleotide-excision repair
inferred from electronic annotation with InterPro:IPR001044
(InterPro Project Members, 2004-)
Cellular Component (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
located_in nucleus
inferred from electronic annotation with InterPro:IPR001044
(InterPro Project Members, 2004-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
mutagen-sensitive 201 (mus201) encodes a protein involved in UV-damage excision repair. [Date last reviewed: 2019-09-19]
Gene Group (FlyBase)
FLAP ENDONUCLEASES -
Flap endonucleases are divalent metal ion-dependent nucleases that catalyze the cleavage of bifurcated nucleic acid structures known as 5'-flaps. (Adapted from PMID:22918592).
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
mus201
Larval survival hypersensitive to exposure to methyl methanesulfonate, nitrogen mustard, and ultraviolet light; weakly hypersensitive to X rays. Female fertility unimpaired, and meiotic disjunction regular. Homozygotes devoid of detectable excision repair (Boyd et al.; Dusenbery, McCormick, and Smith, 1983, Mutat. Res. 112: 215-30); postreplication repair and repair of single-strand breaks induced by X rays normal. Hypermutable to alkylating agents (Smith and Dusenberg, 1988, Mechanisms and Consequences of DNA Damage Processing, Alan R. Liss, Inc., pp. 251-55). Mutant defect rescued by transformation with the endonuclease V gene (denV) of bacteriophage T4 (Banga, Boyd, Valerie, Harris, Kurz, and de Riel, 1989, Proc. Nat. Acad. Sci. USA 86: 3227-31).
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\mus201 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q4U2Q5)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.54

May be component of a dicistronic gene; available data inconclusive (possible low frequency, stage-specific dicistronic transcript).

Gene model reviewed during 5.50

Gene model reviewed during 5.56

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
mus201-RC
FBtr0100473
NM_001032059
3845
1236
mus201-RD
FBtr0346117
NM_001032060
3950
1236
Additional Transcript Data and Comments
Reported size (kB)

3.979 (longest cDNA)

(Houle and Friedberg, 1999)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
mus201-PC
FBpp0099906
139.0
1236
4.99
Q4U2Q5
NP_001027230
AAO41169
mus201-PD
FBpp0311946
139.0
1236
4.99
Q4U2Q5
NP_001027231
AAF52678
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1236 aa isoforms: mus201-PC, mus201-PD
Additional Polypeptide Data and Comments
Reported size (kDa)

1236 (aa)

(Sekelsky et al., 2000, Houle and Friedberg, 1999)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mus201 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.26

Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\mus201 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 14 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of mus201
Transgenic constructs containing regulatory region of mus201
Aberrations (Deficiencies and Duplications) ( 2 )
Inferred from experimentation ( 2 )
Gene disrupted in
Df(2L)N22-14
(Sekelsky et al., 2000)
Gene not disrupted in
Df(2L)N22-5
(Sekelsky et al., 2000)
Inferred from location ( 0 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (6)
Hsap\ERCC5
8 of 14
Yes
Yes
1  
Hsap\BIVM-ERCC5
2 of 14
No
Yes
Hsap\FEN1
2 of 14
No
No
Hsap\GEN1
2 of 14
No
No
1  
Hsap\BIVM
1 of 14
No
Yes
Hsap\EXO1
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (5)
Rnor\Ercc5
8 of 14
Yes
Yes
Rnor\Fen1
2 of 14
No
No
Rnor\Gen1
2 of 14
No
No
Rnor\Bivm
1 of 14
No
Yes
Rnor\Exo1
1 of 14
No
No
Mus musculus (laboratory mouse) (5)
Mmus\Ercc5
7 of 14
Yes
Yes
Mmus\Fen1
2 of 14
No
No
Mmus\Gen1
2 of 14
No
No
Mmus\Bivm
1 of 14
No
Yes
Mmus\Exo1
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (5)
Xtro\ercc5
5 of 13
Yes
Yes
Xtro\fen1
2 of 13
No
No
Xtro\gen1
2 of 13
No
No
Xtro\exo1
1 of 13
No
No
Xtro\LOC101734301
1 of 13
No
Yes
Danio rerio (Zebrafish) (6)
Drer\ercc5
9 of 14
Yes
Yes
Drer\fen1
2 of 14
No
No
Drer\gen1
2 of 14
No
No
Drer\zgc:110269
2 of 14
No
No
Drer\bivm
1 of 14
No
Yes
Drer\exo1
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (4)
Cele\xpg-1
11 of 14
Yes
Yes
Cele\gen-1
3 of 14
No
No
Cele\crn-1
2 of 14
No
No
Cele\exo-1
2 of 14
No
No
Anopheles gambiae (African malaria mosquito) (4)
Agam\AgaP_AGAP008939
10 of 12
Yes
Yes
Agam\AgaP_AGAP002669
2 of 12
No
No
Agam\AgaP_AGAP011448
2 of 12
No
No
Agam\AgaP_AGAP004491
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (6)
Atha\UVH3
10 of 13
Yes
Yes
Atha\AT1G01880
3 of 13
No
No
Atha\AT3G48900
3 of 13
No
No
Atha\AT5G26680
2 of 13
No
No
Atha\AT1G18090
1 of 13
No
No
Atha\AT1G29630
1 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (5)
Scer\RAD2
9 of 13
Yes
Yes
Scer\YEN1
2 of 13
No
No
Scer\DIN7
1 of 13
No
No
Scer\EXO1
1 of 13
No
No
Scer\RAD27
1 of 13
No
No
Schizosaccharomyces pombe (Fission yeast) (4)
Spom\rad13
7 of 12
Yes
Yes
Spom\exo1
1 of 12
No
No
Spom\rad2
1 of 12
No
No
Spom\SPAC12G12.16c
1 of 12
No
No
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:mus201. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (3)
Fen1
4 of 13
Gen
4 of 13
tos
3 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 2 )
    Modifiers Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Interaction
    References
    mus201D1
    ameliorates  autosomal dominant cerebellar ataxia
    modeled by Hsap\ATXN3tr.Q78.UAS.Tag:HA
    (Jung and Bonini, 2007)
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    ERCC5; ERCC excision repair 5, endonuclease
    8 of 14
    133530
     
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Other Interaction Browsers
    Summary of Genetic Interactions
    esyN Network Diagram
    Show/hide secondary interactors 
    (data from AllianceMine provided by esyN)
    esyN Network Key:
    Suppression
    Enhancement
    Other Interaction Browsers
    View in FlyBase Interactions Browser   View in MIST tool (external link)
    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    mus201
    enhanceable
    p53
    (Jassim et al., 2003)
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    p53
    enhanceable
    mus201
    (Jassim et al., 2003)
    External Data
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    MIST (genetic) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    External Data
    Linkouts
    KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2L
    Recombination map
    2-32
    Cytogenetic map
    29C4-29C5
    Sequence location
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    29C4-29C5
    Limits computationally determined from genome sequence between P{lacW}l(2)k03706k03706&P{lacW}Acerk07704 and P{PZ}lmg03424
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    29C-29C
    (Calleja, 1999.6.25)
    29A-29A
    (determined by in situ hybridisation)
    (Louis et al., 1997, EDGP Project Members, 1994-)
    Cytological site tentative; in situ of cosmid 55A11 shows secondary site at 70C.
    (EDGP Project Members, 1994-)
    Experimentally Determined Recombination Data
    Location

    2-32

    (FlyBase, 2016)

    2-27.9

    (Comeron et al., 2012)

    2-34

    (Smith, 1994)

    2-23

    (Boyd et al., 1982)

    2-

    (Boyd et al., 1981)
    Left of (cM)
    (Boyd et al., 1982)
    Right of (cM)
    (Boyd et al., 1982)
    Notes
    Stocks and Reagents
    Stocks (12)
    Genomic Clones (14)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (13)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    BDGP DGC clones
    Other clones
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    Antibody Information
    Laboratory Generated Antibodies
     
    Commercially Available Antibodies
     
    Cell Line Information
    Publicly Available Cell Lines
     
    Other Stable Cell Lines
     
      Other Comments

      Gene is involved in pre-replication DNA repair of UV and AA lesions.

      (de Buendia, 1998)

      Sex-linked recessive lethal (SLRL) test is used to demonstrate inactivation of the nucleotide excision repair (NER) system has a major impact on mutational response of germ cells to mutagens.

      (Vogel and Nivard, 1997)

      Larval survival hypersensitive to exposure to methyl methanesulfonate, nitrogen mustard and ultraviolet light; weakly hypersensitive to X rays. Female fertility unimpaired and meiotic disjunction regular. Homozygotes devoid of detectable excision repair (Boyd et al., 1982; Dusenbery et al., 1983); postreplication repair and repair of single-strand breaks induced by X rays normal. Hypermutable to alkylating agents (Smith and Dusenbery, 1988). Mutant defect rescued by transformation with the endonuclease V gene (denV) of bacteriophage T4 (Banga et al., 1989).

      Relationship to Other Genes
      Source for database merge of

      Source for merge of: mus201 ESTS:55A11T

      (FlyBase, 1996-)
      Additional comments

      Dicistronic annotation CG32956 split out into separate annotations for each open reading frame, CG13399 and CG10890, in release 4.2 of the genome annotation. CG13399 corresponds to Chrac-14 and CG10890 corresponds to mus201.

      (FlyBase Genome Annotators, 2005)

      Source for merge of mus201 ESTS:55A11T was sequence comparison ( date:000505 ).

      (FlyBase, 1996-)
      Nomenclature History
      Source for database identify of

      Source for identity of: mus201 CG10890

      (Burtis, 1999.11)
      Nomenclature comments
      Etymology
      Synonyms and Secondary IDs (15)
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      Study result (0)
      Result
      Result Type
      Title
      External Crossreferences and Linkouts ( 67 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Other crossreferences
      AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
      DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
      EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
      FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
      FlyMine - An integrated database for Drosophila genomics
      InterPro - A database of protein families, domains and functional sites
      KEGG Genes - Molecular building blocks of life in the genomic space.
      MARRVEL_MODEL - MARRVEL (model organism gene)
      Linkouts
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
      DroID - A comprehensive database of gene and protein interactions.
      DRSC - Results frm RNAi screens
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Flygut - An atlas of the Drosophila adult midgut
      iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
      KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
      MIST (genetic) - An integrated Molecular Interaction Database
      References (107)