FlyBase Gene Report: Dmel\blot

General Information

Symbol

Dmel\blot

Species

D. melanogaster

Name

bloated tubules

Annotation Symbol

CG3897

Feature Type

protein_coding_gene

FlyBase ID

FBgn0027660

Gene Model Status

Current

Stock Availability

10 publicly available

Gene Summary

bloated tubules (blot) encodes a member of the sodium- and chloride-dependent neurotransmitter symporter family. It is required for cortical actin organisation and contributes to embryonic cellularization and renal tubule development in larvae. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

74B1-74B2

Sequence location

3L:17,396,031..17,421,514 [-]

Recombination map

3-45

RefSeq locus

NT_037436 REGION:17396031..17421514

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (6 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables neurotransmitter:sodium symporter activity

inferred from sequence model

(Thimgan et al., 2006)

Biological Process (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in amino acid transport

inferred from biological aspect of ancestor with PANTHER:PTN000907004

(GO Reference Genome Project, 2011-)

involved_in neurotransmitter transport

inferred from sequence model

(Thimgan et al., 2006)

involved_in sodium ion transmembrane transport

inferred from biological aspect of ancestor with PANTHER:PTN000177662

(GO Reference Genome Project, 2011-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in membrane

inferred from electronic annotation with InterPro:IPR000175

(InterPro Project Members, 2004-)

inferred from sequence model

(Thimgan et al., 2006)

non-traceable author statement

(Tepass et al., 2001)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000177662

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

SLC6 FAMILY OF SODIUM/CHLORIDE-DEPENDENT NEUROTRANSMITTER TRANSPORTERS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

SLC6 FAMILY OF SODIUM/CHLORIDE-DEPENDENT NEUROTRANSMITTER TRANSPORTERS -: Solute carrier family 6 (SLC6) members are Na⁺/Cl^--dependent transporters of extracellular neurotransmitters, amino acids and osmolytes across the plasma membrane into the cytosol. (Adapted from FBrf0193583).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\blot for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q8IQQ7)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

blot-RA

FBtr0075234

NM_170637

2927

769

blot-RB

FBtr0075233

NM_079401

4054

976

blot-RC

FBtr0075235

NM_170638

3057

809

blot-RD

FBtr0075232

NM_170636

3624

976

blot-RE

FBtr0339898

NM_001300164

3089

809

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

blot-PA

FBpp0074996

86.0

769

9.29

FBpp0074995

107.8

976

9.21

FBpp0074997

91.1

809

9.18

FBpp0074994

107.8

976

9.21

FBpp0308930

91.1

809

9.18

Q8IQQ6

NP_001287093

AHN58118

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

976 aa isoforms: blot-PB, blot-PD

809 aa isoforms: blot-PC, blot-PE

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\blot using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.73

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 4

organism | uniform

(Johnson et al., 1999)

embryonic stage 5

ectoderm | restricted

Comment: anterior cap, 3 broad stripes

(Johnson et al., 1999)

embryonic stage 6

ectoderm | striped

Comment: 6 discontinous stripes

(Johnson et al., 1999)

amnioproctodeal invagination

(Johnson et al., 1999)

embryonic stage 12 -- 13

Malpighian tubule tip cell primordium

(Johnson et al., 1999)

embryonic stage 16

cell | subset of larval ventral nerve cord | segmentally repeated

(Johnson et al., 1999)

antenno-maxillary complex

(Johnson et al., 1999)

embryonic/larval posterior spiracle

(Johnson et al., 1999)

third instar larval stage

Malpighian tubule

• cytoplasm

(Johnson et al., 1999)

adult stage | female oogenesis stage S9 -- S11

nurse cell

(Johnson et al., 1999)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism

(Johnson et al., 1999)

adult stage

adult head

(Romero-Calderon et al., 2007)

adult thorax

Comment: Thorax and abdomen assayed together. Band of 1.9kb detected.

(Romero-Calderon et al., 2007)

adult abdomen

Comment: Thorax and abdomen assayed together. Band of 1.9kb detected.

(Romero-Calderon et al., 2007)

Additional Descriptive Data

blot transcript is detected at all embryonic stages, with highest expression at 0-3 hr AEL and 3-6 hr AEL. blot transcript is evenly distributed in early embryos. At embryonic stage 5 blot transcript is localized to an anterior cap and in three broad ectodermal stripes. At embryonic stage 6 blot transcript is expressed in five strong and one weak ectodermal stripes, which are interrupted by a ventrolateral region of non-expression. There is also a posterior-lateral stripe of expression at the rim of the amnioproctodeal invagination. By stage 13, most blot transcript expression is limited to the Malpighian tubule tip cell primordium. At stage 16, blot transcript is expressed in a segmentally repeated pattern in the ventral nerve cord, in the antennomaxillary complex, and in the posterior spiracles.

(Johnson et al., 1999)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

Reporter: P{lArB}A434

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11

Malpighian tubule primordium

(Johnson et al., 1999)

embryonic stage 12 -- 13

Malpighian tubule tip cell primordium

(Johnson et al., 1999)

embryonic stage 14 -- 15

embryonic Malpighian tubule tip cell

(Johnson et al., 1999)

embryonic stage 16

cell | subset of larval ventral nerve cord | segmentally repeated

(Johnson et al., 1999)

third instar larval stage

embryonic/larval Malpighian tubule

(Johnson et al., 1999)

embryonic/larval central nervous system | restricted

Comment: single cells on surface of brain lobes and ventral nerve cord

(Johnson et al., 1999)

ring gland

(Johnson et al., 1999)

eye-antennal disc

(Johnson et al., 1999)

Reporter: P{PZ}blot⁰¹⁶⁵⁸

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11

Malpighian tubule primordium

(Johnson et al., 1999)

embryonic stage 12 -- 13

Malpighian tubule tip cell primordium

(Johnson et al., 1999)

embryonic stage 14 -- 15

embryonic Malpighian tubule tip cell

(Johnson et al., 1999)

embryonic stage 16

cell | subset of larval ventral nerve cord | segmentally repeated

(Johnson et al., 1999)

third instar larval stage

embryonic/larval Malpighian tubule

(Johnson et al., 1999)

embryonic/larval central nervous system | restricted

Comment: single cells on surface of brain lobes and ventral nerve cord

(Johnson et al., 1999)

ring gland

(Johnson et al., 1999)

eye-antennal disc

(Johnson et al., 1999)

wandering third instar larval stage

imaginal disc

(Meister and Braun, 1995.10)

Malpighian tubule

(Meister and Braun, 1995.10)

adult stage

adult brain | restricted

(Vosshall, 1998.8.10)

lamina

(Vosshall, 1998.8.10)

adult central complex

(Vosshall, 1998.8.10)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\blot in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\blot

View exonic expression by tissue for Dmel\blot

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39939/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0027660

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0027660

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0027660

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3897

Flygut - An atlas of the Drosophila adult midgut

FBgn0027660

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 13 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of blot

Transgenic constructs containing regulatory region of blot

Aberrations (Deficiencies and Duplications) ( 9 )

Inferred from experimentation ( 9 )

Gene disrupted in

Df(3L)81k19

(Johnson et al., 1999)

Df(3L)BSC414

(Christensen et al., 2008.4.15)

Df(3L)BSC415

(Christensen et al., 2008.4.15)

Df(3L)BSC432

(Christensen et al., 2008.4.15)

Df(3L)BSC444

(Christensen et al., 2008.4.15)

Df(3L)Exel6131

(Ryder, 2004.4.26)

Df(3L)st-E23

(Johnson et al., 1999)

Df(3L)tap-74

(Johnson et al., 1999)

Gene not disrupted in

Df(3L)st7

(Johnson et al., 1999)

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | germline clone | non-rescuable maternal effect

lethal | larval stage

lethal | recessive

blot⁰¹⁶⁵⁸

lethal - all die before end of larval stage

blot^M51

viable

blot^EY13445

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

blot^GD2690

viable, with Scer\GAL4^Mef2.PR

blot^GD2690

viable, with Scer\GAL4^pnr-MD237

blot^GD2690

Sterility

Allele

female semi-fertile | germline clone

fertile

blot^EY13445

Other Phenotypes

Allele

abnormal sleep | adult stage, with Scer\GAL4^elav.PLu

blot^KK106850

some die during larval stage

blot^M51

Phenotype manifest in

Allele

denticle

embryo | blastoderm stage | germline clone

Malpighian tubule

ovariole, with Scer\GAL4^tj.PU

blot^KK106850

primordial germ cell | germline clone

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (19)

Hsap\SLC6A15

3 of 14

Yes

Hsap\SLC6A16

3 of 14

Yes

Hsap\SLC6A17

3 of 14

Yes

Hsap\SLC6A18

3 of 14

Yes

Hsap\SLC6A19

3 of 14

Yes

Hsap\SLC6A20

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (18)

Rnor\Slc6a20

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (21)

Mmus\Slc6a20a

3 of 14

Yes

Mmus\Slc6a20b

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (15)

Xtro\lyl1

2 of 13

Yes

Xtro\slc6a8

2 of 13

Yes

Xtro\slc6a15

2 of 13

Yes

Xtro\slc6a17

2 of 13

Yes

Xtro\cdc37

1 of 13

Xtro\cflar

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (33)

Drer\si:ch211-117c9.5

2 of 14

Yes

Drer\si:ch211-132b12.1

2 of 14

Yes

Drer\si:ch211-132b12.3

2 of 14

Yes

Drer\slc6a1b

2 of 14

Yes

Drer\slc6a1l

2 of 14

Yes

Drer\slc6a5

2 of 14

Yes

Drer\slc6a8

2 of 14

Yes

Drer\slc6a9

2 of 14

Yes

Drer\slc6a13

2 of 14

Yes

Drer\slc6a14

2 of 14

Yes

Drer\slc6a16a

2 of 14

Yes

Drer\slc6a16b

2 of 14

Yes

Drer\slc6a18

2 of 14

Yes

Drer\slc6a19a.1

2 of 14

Yes

Drer\slc6a19a.2

2 of 14

Yes

Drer\slc6a19b

2 of 14

Yes

Drer\slc6a22.1

2 of 14

Yes

Drer\slc6a22.2

2 of 14

Yes

Drer\zgc:158427

2 of 14

Yes

Drer\si:ch211-132b12.2

1 of 14

Drer\si:ch211-132b12.6

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (15)

Cele\dat-1

2 of 14

Yes

Cele\mod-5

2 of 14

Yes

Cele\snf-2

2 of 14

Yes

Cele\snf-3

2 of 14

Yes

Cele\snf-5

2 of 14

Yes

Cele\snf-7

2 of 14

Yes

Cele\snf-9

2 of 14

Yes

Cele\snf-11

2 of 14

Yes

Cele\snf-12

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Cele\snf-10

1 of 14

Cele\Y43D4A.1

1 of 14

Anopheles gambiae (African malaria mosquito) (21)

Agam\AgaP_AGAP004932

6 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:blot. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (21)

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

DAT

3 of 13

Gat

3 of 13

GlyT

3 of 13

ine

3 of 13

List

3 of 13

NAAT1

3 of 13

Ntl

3 of 13

SerT

3 of 13

bdg

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 2 )

Human Ortholog

Disease

Evidence

References

SLC6A19; solute carrier family 6 member 19

model of Hartnup disease

IEA

(FlyBase, 2019-)

SLC6A17; solute carrier family 6 member 17

model of autosomal recessive intellectual developmental disorder 48

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SLC6A15; solute carrier family 6 member 15

3 of 14

607971

SLC6A16; solute carrier family 6 member 16

3 of 14

607972

SLC6A17; solute carrier family 6 member 17

3 of 14

610299

INTELLECTUAL DEVELOPMENTAL DISORDER, AUTOSOMAL RECESSIVE 48; MRT48

autosomal recessive intellectual developmental disorder 48

SLC6A18; solute carrier family 6 member 18

3 of 14

610300

SLC6A19; solute carrier family 6 member 19

3 of 14

608893

HARTNUP DISORDER; HND

Hartnup disease

SLC6A20; solute carrier family 6 member 20

3 of 14

605616

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

MIST Molecular Interaction Search Tool

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

65229

DroID - A comprehensive database of gene and protein interactions.

FBgn0027660

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-45

Cytogenetic map

74B1-74B2

Sequence location

3L:17,396,031..17,421,514 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

74B1-74B2

Limits computationally determined from genome sequence between P{PZ}Baldspot⁰²²⁸¹ and P{PZ}blot⁰¹⁶⁵⁸

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

74B-74B

(Johnson, 1999.6.28)

74B1-74B2

(determined by in situ hybridisation)

(Spradling et al., 1999)

74B-74B

(determined by in situ hybridisation)

(Johnson et al., 1999)

74B1-74B2

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

3-45

(FlyBase, 2016)

3-40.7

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (10)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

82696

y¹ v¹; P{TKO.GS05038}attP40

53079

y¹ w^*; Mi{MIC}blot^MI08632

21417

y¹ w^67c23; P{EPgy2}blot^EY13445

34416

y¹ w^*; Mi{MIC}blot^MI01264/TM3, Sb¹ Ser¹

38188

y¹ w^*; Mi{MIC}blot^MI05046/TM3, Sb¹ Ser¹

93964

w^*; TI{CRIMIC.TG4.0}blot^{CR60242-TG4.0}/TM3, Sb¹ Ser¹

16070

y¹ w¹¹¹⁸; PBac{5HPw⁺}blot^A223/TM3, Sb¹ Ser¹

11542

tap⁰¹⁶⁵⁸ P{PZ}blot⁰¹⁶⁵⁸ ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹

VDRC

v101083

P{KK106850}VIE-260B

v5296

w¹¹¹⁸; P{GD2690}v5296

Genomic Clones (14)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (273)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0027660

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0027660

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

blot is required for viability. Mutants show defects in the Malpighian tubule cells of larvae, and the actin filaments in the apical cortex and the cortical cytoskeleton of embryos, and the cellularisation process.

(Johnson et al., 1999)

Relationship to Other Genes

Source for database merge of

Additional comments

Comparison of restriction maps for the genomic regions of tap and blot reveals no overlap. Whole mount in situ hybridization with fragments of the blot walk do not stain tap- expressing cells. tap and blot therefore have distinctive genomic organizations.

(Johnson et al., 1999)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (6)

Reported As

Name Synonyms

Blated tubules

(Sano et al., 2005)

bloated tubules

(Akbari et al., 2014, Thimgan et al., 2006)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 53 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

39939

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q8IQQ7

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q8IQQ7

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39939/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0027660

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0027660

FlyMine - An integrated database for Drosophila genomics

FBgn0027660

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG3897

MARRVEL_MODEL - MARRVEL (model organism gene)

39939

Linkouts

BioGRID - A database of protein and genetic interactions.

65229

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0027660

DroID - A comprehensive database of gene and protein interactions.

FBgn0027660

DRSC - Results frm RNAi screens

FBgn0027660

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0027660

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0027660

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3897

Flygut - An atlas of the Drosophila adult midgut

FBgn0027660

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0027660

References (62)

The following external sites may use different assemblies or annotations than FlyBase.

-0.73

Report Sections