FlyBase Gene Report: Dmel\Agpat1

General Information

Symbol

Dmel\Agpat1

Species

D. melanogaster

Name

1-Acylglycerol-3-phosphate O-acyltransferase 1

Annotation Symbol

CG3812

Feature Type

protein_coding_gene

FlyBase ID

FBgn0030421

Gene Model Status

Current

Stock Availability

9 publicly available

Enzyme Name (EC)

1-acylglycerol-3-phosphate O-acyltransferase (2.3.1.51)

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

AGPAT, AGPAT2

Key Links

Genomic Location

Cytogenetic map

11B16-11C1

Sequence location

X:12,765,266..12,772,851 [+]

Recombination map

1-39

RefSeq locus

NC_004354 REGION:12765266..12772851

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (5 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables 1-acylglycerol-3-phosphate O-acyltransferase activity

inferred from biological aspect of ancestor with PANTHER:PTN000046632

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR004552

(InterPro Project Members, 2004-)

Biological Process (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in phosphatidic acid biosynthetic process

inferred from biological aspect of ancestor with PANTHER:PTN000046632

(GO Reference Genome Project, 2011-)

involved_in phospholipid biosynthetic process

inferred from electronic annotation with InterPro:IPR004552

(InterPro Project Members, 2004-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in endoplasmic reticulum

inferred from biological aspect of ancestor with PANTHER:PTN000046633

(GO Reference Genome Project, 2011-)

located_in membrane

inferred from electronic annotation with InterPro:IPR004552

(InterPro Project Members, 2004-)

Gene Group (FlyBase)

1-ACYLGLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

1-acylglycerol-3-phosphate O-acyltransferase activity

a 1-acyl-sn-glycero-3-phosphate + an acyl-CoA = a 1,2-diacyl-sn-glycero- 3-phosphate + CoA (2.3.1.51)

RHEA 19709:

Summaries

Gene Group (FlyBase)

1-ACYLGLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASES -: 1-acyl-glycerol-3-phosphate O-acyltransferases are involved in the de novo synthesis of triacylglycerol (TAG) and glycerophospholipids, and thus are important for lipid storage/transport and membrane biogenesis. Specifically, they catalyze the second acylation step in TAG synthesis, converting lysophosphatidic acid (LPA) to phosphatidic acid (PA) by transferring an acyl group from fatty acyl-CoA to the sn-2 position of the glycerol backbone. (Adapted from PMID:25415055.)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Agpat1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VYJ4)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.46

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Antisense UTR: 3' UTRs of most extended isoforms overlap significantly the extended 3' UTR of CG32647.

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Agpat1-RA

FBtr0073688

NM_132600

1831

343

Agpat1-RB

FBtr0073689

NM_167337

3795

343

Agpat1-RC

FBtr0333176

NM_001272565

3566

343

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Agpat1-PA

FBpp0073521

38.2

343

10.22

FBpp0073522

38.2

343

10.22

FBpp0305378

38.2

343

10.22

Q9VYJ4

NP_001259494

AGB95336

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

343 aa isoforms: Agpat1-PA, Agpat1-PB, Agpat1-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9VYJ4

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Agpat1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.83

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Agpat1 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Agpat1

View exonic expression by tissue for Dmel\Agpat1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

32230/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0030421

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0030421

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0030421

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3812

Flygut - An atlas of the Drosophila adult midgut

FBgn0030421

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 9 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Agpat1

Transgenic constructs containing regulatory region of Agpat1

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

Agpat1^e03305

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Agpat1^GD3545

viable, with Scer\GAL4^Mef2.PR

Agpat1^GD3545

viable, with Scer\GAL4^pnr-MD237

Agpat1^GD3545

Sterility

Allele

fertile

Agpat1^e03305

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (14)

Hsap\AGPAT1

12 of 14

Yes

Hsap\AGPAT2

11 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (16)

Rnor\Agpat1

13 of 14

Yes

Rnor\Agpat2

10 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (15)

Mmus\Agpat1

13 of 14

Yes

Mmus\Agpat2

10 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (8)

Xtro\agpat1

7 of 13

Yes

Xtro\agpat2

6 of 13

Yes

Xtro\aipl1

1 of 13

Yes

Xtro\ccr2

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (13)

Drer\agpat2

12 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (12)

Cele\acl-2

13 of 14

Yes

Cele\acl-1

11 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (14)

Agam\AgaP_AGAP002021

12 of 12

Yes

Agam\AgaP_AGAP009407

6 of 12

Yes

6 of 12

5 of 12

3 of 12

3 of 12

3 of 12

3 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (15)

Atha\ATS2

8 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (5)

Scer\SLC1

11 of 13

Yes

Scer\CST26

1 of 13

Scer\GPT2

1 of 13

Yes

Scer\SCT1

1 of 13

Yes

Scer\YDR018C

1 of 13

Schizosaccharomyces pombe (Fission yeast) (4)

Spom\slc1

10 of 12

Yes

Spom\SPBC428.14

2 of 12

Spom\SPBC1718.04

1 of 12

Yes

Spom\vps66

1 of 12

Escherichia coli (enterobacterium) (3)

Ecol\plsC

8 of 11

Yes

Ecol\yihG

2 of 11

Ecol\aas

1 of 11

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Agpat1. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (7)

11 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

AGPAT2; 1-acylglycerol-3-phosphate O-acyltransferase 2

model of congenital generalized lipodystrophy type 1

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

AGPAT1; 1-acylglycerol-3-phosphate O-acyltransferase 1

12 of 14

603099

AGPAT2; 1-acylglycerol-3-phosphate O-acyltransferase 2

11 of 14

603100

LIPODYSTROPHY, CONGENITAL GENERALIZED, TYPE 1; CGL1

congenital generalized lipodystrophy type 1

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

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Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Agpat1 - Prosα4

experimental knowledge based

(Guruharsha et al., 2011)

Agpat1 - Prosβ4

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

58622

DroID - A comprehensive database of gene and protein interactions.

FBgn0030421

MIST (protein-protein) - An integrated Molecular Interaction Database

32230

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-39

Cytogenetic map

11B16-11C1

Sequence location

X:12,765,266..12,772,851 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

11B16-11C1

Limits computationally determined from genome sequence between P{EP}EP1632&P{EP}EP1449 and P{EP}EP1461;

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-39

(FlyBase, 2016)

1-40.3

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (9)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

85594

w¹¹¹⁸ PBac{RB}Agpat1^e04307

53437

y¹ w^* Mi{MIC}Agpat1^MI09991

13373

y¹ w^67c23 P{Mae-UAS.6.11}Agpat1^GG01218

600024

y¹ w^* TI{CRIMIC.TG4.2}Agpat1^{CR70957-TG4.2}

11192

11271

HMJ21807

y¹ v¹; P{TRiP.HMJ21807}attP40/CyO

VDRC

v109657

P{KK102214}VIE-260B

v44418

w¹¹¹⁸; P{GD3545}v44418/TM3

Genomic Clones (9)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (136)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD44987

Other clones

DPiM_LD44987

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0030421

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0030421

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Agpat1 CG3812

(Wilfling et al., 2013)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

AGPAT

(Lehmann, 2021, Thanh et al., 2020)

AGPAT1

(Heier et al., 2021, Wilfling et al., 2013)

AGPAT2

(Lehmann, 2018, Tian et al., 2011)

Agpat1

(Wat et al., 2020, Gene Disruption Project members, 2018-)

CG3812

(National Institute of Genetics Fly Stocks, 2023-, Toprak et al., 2020, Story et al., 2019, Vartiainen et al., 2014, Wilfling et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Kuhnlein, 2012, Guruharsha et al., 2011, Keleman et al., 2009.8.5, Legube et al., 2006, Lipatov et al., 2005)

DmAGPAT1/2

(Kuhnlein, 2012)

dAGPAT1

(Thanh et al., 2020)

Name Synonyms

1-Acylglycerol-3-phosphate O-acyltransferase 1

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 61 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

32230

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VYJ4

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q9VYJ4

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VYJ4

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

32230/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0030421

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0030421

FlyMine - An integrated database for Drosophila genomics

FBgn0030421

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG3812

MARRVEL_MODEL - MARRVEL (model organism gene)

32230

Linkouts

BioGRID - A database of protein and genetic interactions.

58622

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0030421

DroID - A comprehensive database of gene and protein interactions.

FBgn0030421

DRSC - Results frm RNAi screens

FBgn0030421

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0030421

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0030421

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3812

Flygut - An atlas of the Drosophila adult midgut

FBgn0030421

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0030421

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (protein-protein) - An integrated Molecular Interaction Database

32230

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

References (50)

The following external sites may use different assemblies or annotations than FlyBase.

-0.83

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