FlyBase Gene Report: Dmel\Gcat

General Information

Symbol

Dmel\Gcat

Species

D. melanogaster

Name

Glycine C-acetyltransferase

Annotation Symbol

CG10361

Feature Type

protein_coding_gene

FlyBase ID

FBgn0036208

Gene Model Status

Current

Stock Availability

5 publicly available

Enzyme Name (EC)

glycine C-acetyltransferase (2.3.1.29)

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

68E1-68E1

Sequence location

3L:11,798,948..11,800,861 [-]

Recombination map

3-37

RefSeq locus

NT_037436 REGION:11798948..11800861

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (5 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables glycine C-acetyltransferase activity

inferred from electronic annotation with InterPro:IPR011282

(InterPro Project Members, 2004-)

enables pyridoxal phosphate binding

inferred from electronic annotation with InterPro:IPR004839

(InterPro Project Members, 2004-)

Biological Process (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in biosynthetic process

inferred from electronic annotation with InterPro:IPR004839

(InterPro Project Members, 2004-)

involved_in threonine catabolic process

inferred from electronic annotation with InterPro:IPR011282

(InterPro Project Members, 2004-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in mitochondrion

inferred from biological aspect of ancestor with PANTHER:PTN008550589

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

C-ACETYLTRANSFERASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

glycine C-acetyltransferase activity

acetyl-CoA + glycine = (2S)-2-amino-3-oxobutanoate + CoA (2.3.1.29)

RHEA 20736:

Summaries

Gene Group (FlyBase)

C-ACETYLTRANSFERASES -: C-acetyltransferases catalyze the transfer of an acetyl group to a carbon atom on the acceptor molecule.

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Gcat for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VTN9)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.45

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Gcat-RA

FBtr0076084

NM_140252

1431

417

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Gcat-PA

FBpp0075815

45.5

417

8.35

Q9VTN9

NP_648509

AAF50007

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Gcat using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG10361_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.40

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 13 -- 16

dorsal pharyngeal muscle cell

(Fisher et al., 2012)

dorsal sensory complex proper primordium

Comment: reported as dorsal/lateral sensory complexes

(Fisher et al., 2012)

embryonic dorsal epidermis

(Fisher et al., 2012)

embryonic head sensory system

(Fisher et al., 2012)

embryonic ventral epidermis

(Fisher et al., 2012)

embryonic/larval somatic muscle cell

(Fisher et al., 2012)

sensory nervous system primordium

(Fisher et al., 2012)

ventral sensory complex primordium

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Gcat in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Gcat

View exonic expression by tissue for Dmel\Gcat

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0036208

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39333/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0036208

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0036208

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0036208

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10361

Flygut - An atlas of the Drosophila adult midgut

FBgn0036208

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Gcat

Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 2 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Gcat

Transgenic constructs containing regulatory region of Gcat

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

Gcat^e04492

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Gcat^GD6956

viable, with Scer\GAL4^Mef2.PR

Gcat^GD6956

viable, with Scer\GAL4^pnr-MD237

Gcat^GD6956

viable, with Scer\GAL4^tin.CΔ4

Gcat^GD6956

Sterility

Allele

fertile

Gcat^e04492

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (8)

Hsap\GCAT

14 of 14

Yes

5 of 14

5 of 14

3 of 14

3 of 14

3 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (8)

Rnor\Gcat

13 of 14

Yes

5 of 14

5 of 14

3 of 14

3 of 14

3 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (8)

Mmus\Gcat

14 of 14

Yes

6 of 14

5 of 14

3 of 14

3 of 14

2 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (8)

Xtro\gcat

8 of 13

Yes

4 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (9)

Drer\gcat

14 of 14

Yes

6 of 14

6 of 14

3 of 14

3 of 14

3 of 14

2 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (5)

Cele\T25B9.1

14 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (5)

Agam\AgaP_AGAP005781

12 of 12

Yes

5 of 12

3 of 12

3 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (12)

Atha\BIOF

11 of 13

Yes

3 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (5)

Scer\HEM1

5 of 13

Yes

2 of 13

2 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (5)

Spom\hem1

5 of 12

Yes

2 of 12

2 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (3)

Ecol\kbl

9 of 11

Yes

Ecol\bioF

5 of 11

Yes

Ecol\glyA

1 of 11

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Gcat. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (4)

9 of 13

5 of 13

5 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Alzheimer disease, susceptibility to (postulated), GCAT-related

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Gcat^HMC02902

exacerbates Alzheimer's disease

modeled by Hsap\APP^2xAPP.SP.UAS

(Kizil et al., 2022)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

GCAT; glycine C-acetyltransferase

14 of 14

607422

ALAS1; 5'-aminolevulinate synthase 1

5 of 14

125290

ALAS2; 5'-aminolevulinate synthase 2

5 of 14

301300

SPTLC1; serine palmitoyltransferase long chain base subunit 1

3 of 14

605712

SPTLC2; serine palmitoyltransferase long chain base subunit 2

3 of 14

605713

NEUROPATHY, HEREDITARY SENSORY AND AUTONOMIC, TYPE IC; HSAN1C

hereditary sensory and autonomic neuropathy type 1C

SPTLC3; serine palmitoyltransferase long chain base subunit 3

3 of 14

611120

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

MIST Molecular Interaction Search Tool

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0036208

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

L-threonine degradation II

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-37

Cytogenetic map

68E1-68E1

Sequence location

3L:11,798,948..11,800,861 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

68E1-68E1

Limits computationally determined from genome sequence between P{PZ}l(3)01239⁰¹²³⁹&P{lacW}l(3)01239^j9B4 and P{PZ}CycA⁰²⁴⁶¹&P{PZ}CycA⁰³⁹⁴⁶

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-37

(FlyBase, 2016)

3-30.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (5)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

18265

w¹¹¹⁸; PBac{RB}Gcat^e04492

24299

w¹¹¹⁸; Mi{ET1}Gcat^MB04239

44512

y¹ sc^* v¹ sev²¹; P{TRiP.HMC02902}attP2

NIG-Fly

HMC02902

y¹ sc^* v¹ sev²¹; P{TRiP.HMC02902}attP2

VDRC

v16034

w¹¹¹⁸; P{GD6956}v16034

Genomic Clones (9)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (28)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE16411

Other clones

FI03238

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0036208

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0036208

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Gcat CG10361

(FlyBase, 2017-)

Nomenclature comments

Etymology

Named 'Gcat' based on the vertebrate ortholog.

(FlyBase, 2017-)

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG10361

(Fisher et al., 2023, Deshpande et al., 2022, Ding et al., 2022, Lindsey et al., 2021, Palu and Thummel, 2016, Shih et al., 2015, Japanese National Institute of Genetics, 2012.5.21, Cammarato et al., 2011, Kong et al., 2010, Ni et al., 2010.12.1)

GCAT

(Kizil et al., 2022)

Gcat

(Kizil et al., 2022)

Name Synonyms

Glycine C-acetyltransferase

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 38 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

39333

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VTN9

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VTN9

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0036208

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39333/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0036208

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0036208

FlyMine - An integrated database for Drosophila genomics

FBgn0036208

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG10361

MARRVEL_MODEL - MARRVEL (model organism gene)

39333

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0036208

DroID - A comprehensive database of gene and protein interactions.

FBgn0036208

DRSC - Results frm RNAi screens

FBgn0036208

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG10361_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0036208

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0036208

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

L-threonine degradation II

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10361

Flygut - An atlas of the Drosophila adult midgut

FBgn0036208

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

References (34)

The following external sites may use different assemblies or annotations than FlyBase.

-0.40

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