FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Rbf, Dp
FlyBase ID
FBig0000094915
Interaction Network
Interactions Browser links
Rbf network
Dp network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0087034-3.coIP.WB
Reference
Du et al., 1996
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Schneider's-line-2
Participants
Corresponds to
Reported as
Role
Note
Dp 

dDP

bait
Rbf 

RBF

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait endogenous to embryonic extract.

(Du et al., 1996)

Prey endogenous to embryonic extract.

(Du et al., 1996)
FBrf0161049-3.coIP.WB
Reference
Weng et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Dp 

dDP

bait
Rbf 

RBF

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic extracts; bait produced from endogenous gene; prey produced from endogenous gene.

(Weng et al., 2003)
FBrf0180022-1.coIP.WB
Reference
Korenjak et al., 2004
Description
physical association
Assay
Collection
Source/Stage
embryo / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rbf 

RBF1

bait
Dp 

dDP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract; bait produced from endogenous gene; prey produced from endogenous gene.

(Korenjak et al., 2004)

Precipitations were done in the presence of ethidium bromide to rule out DNA mediated protein protein interaction.

(Korenjak et al., 2004)
FBrf0180022-14.coIP.WB
Reference
Korenjak et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Rbf 

RBF1

bait

FLAG tag

Dp 

dDP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Korenjak et al., 2004)

Precipitations were done in the presence of ethidium bromide to rule out DNA mediated protein protein interaction.

(Korenjak et al., 2004)
FBrf0180538-8.coIP.WB
Reference
Taylor-Harding et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Schneider's-line-2
Participants
Corresponds to
Reported as
Role
Note
Rbf 

RBF1

bait

Flag tag

Dp 

DP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of SL2 cells; bait produced from transfected construct; prey produced from endogenous gene.

(Taylor-Harding et al., 2004)

Positive control

(Taylor-Harding et al., 2004)
FBrf0192129-2.coIP.WB
Reference
Shibutani et al., 2007
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 12blastoderm stagegastrula stageembryonic stage 8embryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3embryonic stage 4embryonic stage 2embryonic cycle 9embryonic stage 5embryonic stage 11embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 4embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rbf 

Rbf1

bait
Dp 

Dp

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Shibutani et al., 2007)
FBrf0227292-500.TFIN
Reference
Rhee et al., 2014
Description
physical association
Assay
Collection
TF_Interaction_Network-2
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Dp 

FBgn0011763

unspecified role
Dp 

FBgn0011763

unspecified role
Dp 

FBgn0011763

unspecified role
Rbf 

FBgn0015799

unspecified role
Rbf 

FBgn0015799

unspecified role
Rbf 

FBgn0015799

unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; proteins produced from transfected construct or endogenous gene.

(Rhee et al., 2014)

HGScore = 23.37099

(Rhee et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (6)