FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
kirre, sns
FlyBase ID
FBig0000097361
Interaction Network
Interactions Browser links
kirre network
sns network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0139639-1.BA.FM
Reference
Dworak et al., 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
kirre 

Duf

neutral component
kirre 

Duf

neutral component
kirre 

Duf

neutral component
sns 

SNS

neutral component
sns 

SNS

neutral component
sns 

SNS

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was intact S2 cells; proteins produced from transfected constructs.

(Dworak et al., 2001)

S2 cells expressing sns were mixed with S2 cells expressing kirre : each cell type was labeled by a different dye. The formation of cell clusters containing both kirre and sns expressing cells was assayed by fluorescence microscopy.

(Dworak et al., 2001)
FBrf0180484-1.BA.FM
Reference
Galletta et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
sns 

SNS

bait
kirre 

Duf/Kirre

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was intact S2 cells; bait produced from transfected construct; prey produced from transfected construct.

(Galletta et al., 2004)

S2 cells expressing sns were mixed with S2 cells expressing kirre, and the formation of cell clusters containing both kirre and sns expressing cells was assayed by immunofluorescence.

(Galletta et al., 2004)
FBrf0180484-3.coIP.WB
Reference
Galletta et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
kirre 

Duf/Kirre

bait

HA tag

sns 

SNS

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Galletta et al., 2004)
FBrf0207479-5.BA.FM
Reference
Shelton et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
sns 

sns

neutral component
sns 

sns

neutral component
sns 

sns

neutral component
kirre 

kirre

neutral component
kirre 

kirre

neutral component
kirre 

kirre

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live S2 cells; proteins produced from transfected constructs.

(Shelton et al., 2009)

Physical interaction of proteins expressed on the surface of cells is inferred from the aggregation of live cells.

(Shelton et al., 2009)
FBrf0211429-2.coIP.WB
Reference
Bao et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
kirre 

kirre

bait

FLAG tag

sns 

sns

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Bao et al., 2010)
FBrf0222867-182.ELISA
Reference
Ozkan et al., 2013
Description
physical association
Assay
Collection
ECIA
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
sns 

sns

bait

Fc tag

sns 
kirre 

kirre

prey

alkaline phosphatase tag, COMP pentamerization motif

kirre 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
sns 
extracellular domain
sufficient binding region
kirre 
extracellular domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait derived from conditioned media of transfected S2 cell line; prey derived from conditioned media of transfected S2 cell line.

(Ozkan et al., 2013)
FBrf0222867-96.ELISA
Reference
Ozkan et al., 2013
Description
physical association
Assay
Collection
ECIA
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
kirre 

kirre

bait

Fc tag

kirre 
sns 

sns

prey

alkaline phosphatase tag, COMP pentamerization motif

sns 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
kirre 
extracellular domain
sufficient binding region
sns 
extracellular domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait derived from conditioned media of transfected S2 cell line; prey derived from conditioned media of transfected S2 cell line.

(Ozkan et al., 2013)
FBrf0253179-2.ELISA
Reference
Bali et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sns 

Sns

bait

alkaline phosphatase tag

kirre 

Kirre

prey

mi3 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait derived from transfected human Expi293 cell line; prey derived from transfected human Expi293 cell line.

(Bali et al., 2022)
FBrf0253179-3.ELISA
Reference
Bali et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sns 

Sns

bait

alkaline phosphatase tag

kirre 

Kirre

prey

Fc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait derived from transfected human Expi293 cell line; prey derived from transfected human Expi293 cell line.

(Bali et al., 2022)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (6)