Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

stck, parvin, Ilk and ics copurified stoichiometrically.

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

stck, parvin, Ilk and ics copurified stoichiometrically.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Pulldown performed under native conditions using an Ni-NTA agarose gel.

The Q38A mutation of stck does not interfere with binding to ics

Interaction in vitro; bait produced as transgenic fusion protein; prey derived from S2 cell extract.

The Q38A mutation of stck does not interfere with binding to ics

Source was adults of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

Two-hybrid system: yeast

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was library of randomly mutagenized cDNA).

One of three stck interactions observed in two embryo replicates and in S2 cells.

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

One of three stck interactions observed in two embryo replicates and in S2 cells.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

One of three stck interactions observed in two embryo replicates and in S2 cells.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.