Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast LexA-BD/B42-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast LexA-BD/B42-AD
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from embryonic extract.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2R+ cell extract.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from Clone8 cell extract.
V43E, not V43Q ("mut4")
K46V, Q47A ("mut6")
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
