Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
Interaction in vitro; bait derived from transfected S2 cell lysate; prey produced as a recombinant fusion protein in bacterial system.
Embryonic extract was treated with RNase to demonstrate that the interaction is RNA independant.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.
