FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
edl, aop
FlyBase ID
FBig0000113404
Interaction Network
Interactions Browser links
edl network
aop network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0135901-1.Y2H
Reference
Baker et al., 2001
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aop 

Yan

bait

fused to GAL4 DNA-binding domain

edl 

Mae

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Baker et al., 2001)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Baker et al., 2001)
FBrf0135901-2.PD.A
Reference
Baker et al., 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aop 

Yan

bait

GST tag

aop 
aop 
edl 

Mae

prey

[35]S label

edl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
aop 
Pnt domain
necessary binding region
aa 46-107
aop 
Pnt domain residue 84
mutation disrupting interaction
aa 84

G84P

edl 
interaction domain residue 139
mutation disrupting interaction
aa 139

G139P

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Baker et al., 2001)
FBrf0135901-6.coIP.WB
Reference
Baker et al., 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aop 

Yan

bait

FLAG tag

edl 

Mae

prey

MYC tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of COS-7 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Baker et al., 2001)

rl does not precipitate with aop in this experiment.

(Baker et al., 2001)
FBrf0157239-1.coIP.WB
Reference
Tootle et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
edl 

MAE

bait

MYC tag

aop 

YAN

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Tootle et al., 2003)

The amount of aop bound to edl is reduced in RasV12 stimulated cells.

(Tootle et al., 2003)
FBrf0161068-2.PD.A
Reference
Yamada et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aop 

YAN

bait

GST tag

edl 

edl

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Yamada et al., 2003)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)