pVALIUM10 allows cloning of a hairpin product downstream of UAS sequences for GAL4-inducible expression; contains v^+t1.8 as a selectable marker; contains two introns: a ftz intron, located between the inverted DNA repeats, and another ftz intron between the hairpin product and the SV40 polyA tail to facilitate hairpin-RNA processing and export from the nucleus; two pentamers of UAS sites, one of which is flanked by loxP sites and can thus be excised using the P1cre recombinase to generate a 5xUAS derivative; the hsp70 basal promoter; two gypsy insulator sequences flanking the transcription unit to enhance hairpin DNA transcription; an attB sequence to allow phiC31:int-targeted integration at genomic attP landing sites.

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