From j.roote@XXXX Wed Feb 06  13:43:02  2002
To: rd120@XXXX
Subject: Df kit
rachel,
We have made the following deletions:
deletion LH insert scaffold(A) RH insert scaffold(B) cyt (inferred) kb(B-A)
Df(2R)ED1 P{RS3r}CB-0140-3 11989287 P{RS5r}5-HA-1118 12059882 53F1; 53F11
70595
Df(3R)ED2 P{RS3r}CB-0160-3 14158440 P{RS5r}5-HA-1087 14856038 91A2;91F2
697598
Df(2L)ED3 P{RS5r}5-HA-1150 14377917 P{RS3r}CB-0183-3 15218480 35A;35D 840563
Genetic evidence:
ED1 lethal with Df(2R)P803-Δ15 and In(2LR)DTD99. Note also that
DTD99 is lethal over P803-delta15.
ED2 lethal with (TBA)
ED3 is el+, noc-,osp-, nht-, esg+, enhances Scutoid phenotype.
verification by sequence is TBA. I may have mentioned that there is
an inherent problem with sequencing these Dfs \- they have two 3' P
ends (i.e. there is 3' P at both ends of the reconstituted element).
J