From kcook@XXXX Mon Jun 02  16:46:50  2003
To: flybase-updates@XXXX
Subject: Isolation and characterization of Df(3L)BSC35
Isolation and characterization of Df(3L)BSC35
Rachel Andrade and Kevin Cook
Bloomington Drosophila Stock Center, Indiana University
Df(3L)BSC35 was isolated as a P transposase-induced male recombination
event involving P{EP}smgEP3556 and P{lacW}l(3)j2B9j2B9. The deletion
was isolated as a rhove-1-e1 recombinant chromosome from the cross
rhove-1 pp e1 females x CyO, H{PDelta2-3}HoP2.1/+; rhove-1
P{EP}smgEP3556/P{lacW}l(3)j2B9j2B9 e1 males. The mini-white marker
from at least one insertion was retained on the deletion
chromosome. Polytene chromosome squashes showed the breakpoints
66F1-2;67B2-3 (the distal breakpoint fell within the 66F1,2 doublet band,
leaving a portion of distal 66F1,2 undeleted). Df(3L)BSC35 failed to
complement Rdl1, P{PZ}bol1 and P{lacW}aayS042314.
__________________________________________________________
Kevin Cook, Ph.D. Bloomington Drosophila Stock Center
Department of Biology http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University 812-855-5782
1001 E. Third St. 812-855-2577 (fax)
Bloomington, IN 47405-3700 kcook@XXXX