FlyBase Insertion Report: Dmel\P{PZ}bol[1]

General Information

Symbol

Dmel\P{PZ}bol¹

Species

D. melanogaster

Name

FlyBase ID

FBti0003430

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

ms(3)07272

Affected gene(s)

bol, Ecol\lacZ

Viability / fertility

Associated allele(s)

bol¹, Ecol\lacZ^bol-1

Stock availability

2 publicly available

Genomic Location

Chromosomal Location

3L ( 66F6 )

Sequence Location

3L:9,125,399..9,125,399 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

3L:9,125,399..9,125,399 [+]

(FlyBase, 2005)

Orientation

Cytological location (computed by FlyBase)

66F6 (inferred by FlyBase from sequence location)

Cytological location (reported)

66F1-66F6 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AQ026435

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

bol

(Fingerhut et al., 2019, Hoopfer et al., 2008, Sofola et al., 2007, Castrillon et al., 1993)

Ecol\lacZ

(Castrillon et al., 1993)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

bol¹

Phenotypic Class Phenotype Manifest in Detailed Description

Ecol\lacZ^bol-1

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

In wandering third instar larva, no detectable β-gal staining.

(Meister and Braun, 1995.10)

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

ms(3)07272

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (2)

Bloomington

11794

P{PZ}bol¹ ry⁵⁰⁶/TM6B, Tb¹

Kyoto

101719

P{PZ}bol¹ ry⁵⁰⁶ / TM3, ry^RK Sb¹

External Crossreferences and Linkouts ( 0 )

Comments

Location 3L:9099345-9099346 determined by FlyBase alignment of dbGSS accession AQ026435 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.

(FlyBase, 2005)

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

P(PZ)bol¹

(Fingerhut et al., 2019)

P1794

(Meister and Braun, 1995.10)

P{PZ}bol¹

(Hoopfer et al., 2008, FlyBase, 2005, Choe et al., 2002, Castrillon et al., 1993, FlyBase, 1992-)

P{PZ}ms(3)07272⁰⁷²⁷²

(FlyBase, 1992-)

P{ry^+t7.2=PZ}bol¹

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

FBti0000215

References (17)

Report Sections

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