Date: Tue, 29 Aug 2006  15:37:09  -0400
To: flybase-updates@XXXX
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(2R)BSC161
Cc: kaufmanXXXX, mdealXXXX, Stacey Christensen <sjchristXXXX>, Jill Gresens <jgresensXXXX>
Isolation and characterization of Df(2R)BSC161
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC161 was isolated as a FLP recombinase-induced recombination 
event involving PBac{WH}mbl[f06658] and P{XP}CG30104[d00824]. The 
deletion was isolated as a chromosome lacking miniwhite markers in 
progeny of P{hsFLP}1, y[1] w[1118]; 
PBac{WH}mbl[f06658]/P{XP}CG30104[d00824] males crossed to w[1118]; 
P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat shocked as 
larvae as described in Parks et al., Nature Genetics 36: 288-292, 
2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.WH5}BSC161 from the segment of 
PBac{WH}mbl[f06658] to the left of its FRT site and the segment of 
P{XP}CG30104[d00824] to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
The cytological breakpoints of Df(2R)BSC161 predicted from the 
Release 4 genomic coordinates of the transposable element insertion 
sites are 54B2;54B17. It failed to complement cnk[k16314].
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX