From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updatesXXXX, Stacey Christensen <sjchristXXXX>
Subject: 	Isolation and characterization of Df(2L)BSC354
Date: 	Mon, 29 Oct 2007  12:07:19  -0400  ( 16:07  GMT)
Isolation and characterization of Df(2L)BSC354
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC354 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}d11570 and PBac{WH}f07568. The deletion was 
isolated as a chromosome lacking miniwhite markers in progeny of 
P{hsFLP}1, y[1] w[1118]; P{XP}d11570/PBac{WH}f07568 males crossed to 
w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat 
shocked as larvae as described in Parks et al., Nature Genetics 36: 
288-292, 2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.WH5}BSC354 from the segment of P{XP}d11570 
to the left of its FRT site and the segment of PBac{WH}f07568 to the 
right of its FRT site. Its presence was verified using the PCR 
methods and primers described in Parks et al. The cytological 
breakpoints of Df(2L)BSC354 predicted from the Release 5 genomic 
coordinates of the transposable element insertions sites are 
26D7;26E3. It failed to complement eya[cli-IID] and eya[2].
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX