From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updatesXXXX, Stacey Christensen <sjchristXXXX>
Subject: 	Isolation and characterization of Df(2R)BSC330
Date: 	Mon, 29 Oct 2007  12:07:17  -0400  ( 16:07  GMT)
Isolation and characterization of Df(2R)BSC330
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC330 was isolated as a FLP recombinase-induced recombination 
event involving PBac{WH}CG10253[f02060] and P{XP}d05873. The deletion 
was isolated as a chromosome lacking miniwhite markers in progeny of 
P{hsFLP}1, y[1] w[1118]; PBac{WH}CG10253[f02060]/P{XP}d05873 males 
crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males 
were heat shocked as larvae as described in Parks et al., Nature 
Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in 
preceding and succeeding generations maintained the original genetic 
background of the Exelixis insertion stocks (Thibault et al., Nature 
Genetics 36: 283-287, 2004; FBrf0175002). The recombination event 
generated the genetic element P+PBac{XP5.WH5}BSC330 from the segment 
of PBac{WH}CG10253[f02060] to the left of its FRT site and the 
segment of P{XP}d05873 to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
The cytological breakpoints of Df(2R)BSC330 predicted from the 
Release 5 genomic coordinates of the transposable element insertions 
sites are 51D3;51F9. It failed to complement aPKC[k06403] and scb[2]. 
Df(2R)FDD-0284964 is a synonym for Df(2R)BSC330.
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX