FlyBase Allele Report: Dmel\scb[2]

General Information

Symbol

Dmel\scb²

Species

D. melanogaster

Name

FlyBase ID

FBal0015261

Feature type

allele

Associated gene

Dmel\scb

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage | somatic clone

(Lin et al., 2013)

decreased cell number | adult stage | somatic clone

(Lin et al., 2013)

lethal (with Df(2R)BSC346)

(Kahsai and Cook, 2018)

Phenotype Manifest In

Detailed Description

Statement

Reference

Adult-generated scb² homozygous mutant intestinal stem cell clones have reduced maintenance 7 days and 14 days after clone induction compared to control clones; remaining clones contain fewer cells by day 14; enteroendocrine cell/enterocyte ratio is unaffected and intestinal stem cells show no obvious signs of apoptosis.

(Lin et al., 2013)

Mutant embryos show a reduced level of phagocytosis of apoptotic cells compared to controls.

(Nonaka et al., 2013)

The cardioblasts of the heart region of the dorsal vessel in homozygous stage embryos show a less regular alignment of their nuclei along the midline in mutant embryos compared to wild type. No lumen develops between contralaterally apposed cardioblasts in the heart region of mutant embryos, in contrast to wild type. The leading edge membrane of migrating mutant cardioblasts shows less vigorous activity than that of wild-type cardioblasts.

(Vanderploeg et al., 2012)

Mutant embryos have no severe defects in salivary gland shape or positioning.

(Bradley et al., 2003)

Germ-line clones exhibit a U shaped embryonic phenotype at low penetrance (about 4.5%) suggesting germ band retraction defects. scb^5J38/scb² embryos also exhibit a U shaped embryonic phenotype at low penetrance (about 4%). scb²/Df(2R)Jp1 show the same phenotype at 11% penetrance.

(Schock and Perrimon, 2003)

The germ band twists laterally, rather than extending dorsally as in wild type. Proper orientation of the germ band in recovered by the completion of germ-band extension. Embryos exhibit mislocalisation of the pericardial cells and there are fewer of these cells than in wild type. Embryos exhibit significant gaps in the dorsal trunk of the trachea. Embryos frequently display one salivary gland misshapen and smaller than the other, the gland may also be shifted closer to the midline.

(Stark et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

scb²/scb[+] is an enhancer of visible | dominant phenotype of sog^EP7

(Araujo et al., 2003)

scb²/scb[+] is an enhancer of visible | dominant phenotype of sog^EP11

(Araujo et al., 2003)

Suppressor of

Statement

Reference

scb²/scb[+] is a suppressor of decreased occurrence of cell division phenotype of CycE^JP

(Brumby et al., 2004)

NOT Suppressor of

Statement

Reference

scb² is a non-suppressor of lethal | pupal stage phenotype of Csk^j1D8/Csk^S030003

(Read et al., 2004)

Other

Statement

Reference

CAP^49e, scb² has abnormal cell number | embryonic stage phenotype

(Jammrath et al., 2020)

Fak^N30/Fak^KG00304, scb²/scb[+] has abnormal neuroanatomy | third instar larval stage phenotype

(Tsai et al., 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

scb² has germ band | germline clone phenotype, enhanceable by wb^4Y18

(Schock and Perrimon, 2003)

scb² has embryo | germline clone phenotype, enhanceable by wb^4Y18

(Schock and Perrimon, 2003)

Enhancer of

Statement

Reference

scb²/scb[+] is an enhancer of wing vein phenotype of sog^EP7

(Araujo et al., 2003)

scb²/scb[+] is an enhancer of wing vein phenotype of sog^EP11

(Araujo et al., 2003)

scb² is an enhancer of germ band | germline clone phenotype of wb^4Y18

(Schock and Perrimon, 2003)

scb² is an enhancer of embryo | germline clone phenotype of wb^4Y18

(Schock and Perrimon, 2003)

Suppressor of

Statement

Reference

scb² is a suppressor of eye disc & S phase phenotype of CycE^JP

(Brumby et al., 2004)

scb²/scb[+] is a suppressor of eye phenotype of CycE^JP

(Brumby et al., 2004)

Other

Statement

Reference

CAP^49e, scb² has embryonic cardioblast phenotype

(Jammrath et al., 2020)

LanA^9-32, scb²/scb[+] has embryonic heart cardioblast phenotype

(Vanderploeg et al., 2012)

mys¹, scb²/scb[+] has embryonic heart cardioblast phenotype

(Vanderploeg et al., 2012)

rhea¹, scb²/scb[+] has embryonic heart cardioblast phenotype

(Vanderploeg et al., 2012)

scb²/scb[+], wb^SF11 has embryonic heart cardioblast phenotype

(Vanderploeg et al., 2012)

scb²/scb[+], vkg^p1003-83 has embryonic heart cardioblast phenotype

(Vanderploeg et al., 2012)

Dab^M54-R, scb²/scb[+] has embryonic heart cardioblast phenotype

(Vanderploeg et al., 2012)

Fak^N30/Fak^KG00304, scb²/scb[+] has NMJ bouton | increased number | third instar larval stage phenotype

(Tsai et al., 2008)

Fak^N30/Fak^KG00304, scb²/scb[+] has neuromuscular junction | third instar larval stage phenotype

(Tsai et al., 2008)

mew^M6, scb² has embryonic/larval midgut | embryonic stage phenotype

(Martin-Bermudo et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

mys¹/+ ; scb²/+ and vkg^p1003-83/scb² double heterozygous embryos show defects in continuity and alignment of the cardioblasts at the dorsal midline.

LanA^9-32/+ ; scb²/+ and wb^SF11/scb² double heterozygous embryos show defects in alignment of the cardioblasts at the dorsal midline.

(Vanderploeg et al., 2012)

Significant overgrowth of the neuromuscular junction (NMJ) (increased bouton number per muscle area and increased NMJ length per muscle area) is seen in scb²/+ Fak^N30/Fak^KG00304 third instar larvae.

(Tsai et al., 2008)

Suppresses the S phase defects seen in the CycE^JP eye disc.

(Brumby et al., 2004)

Salivary glands in mew^M6 scb² mutant embryos are indistinguishable from those in mew^M6 embryos.

(Bradley et al., 2003)

Heterozygosity for scb² does not result in lethality in Abl¹/+ embryos derived from homozygous Abl¹ female germline clones.

(Grevengoed et al., 2001)

if^B4 scb² double mutant embryos show no difference in midgut development compared to if^B4 single mutant embryos. mew^M6 scb² double mutant embryos show a strong defect in the migration of the endodermal midgut cells, with a delay in migration of approximately 2 hours (similar to that observed in embryos lacking both maternal and zygotic mys function).

(Martin-Bermudo et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

scb^62S9

(Brumby et al., 2004)

scb^E93

(Brumby et al., 2004)

Partially rescued by

scb² is partially rescued by scb^UAS.cVa/Scer\GAL4^Mef2.PR

(Vanderploeg et al., 2012)

Comments

Expression of scb^Scer\UAS.cVa under the control of Scer\GAL4^Mef2.PR partially restores lumen formation between cardioblasts in the heart region of scb²/scb² embryos.

(Vanderploeg et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

68157

P{neoFRT}42D scb²/CyO

3098

cn¹ scb² bw¹ speck¹/CyO

Kyoto

106839

cn¹ scb² bw¹ speck¹/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

scab^IIG

(Arora et al., 1995)

scb²

(Jammrath et al., 2020, Kahsai and Cook, 2018, Lin et al., 2013, Nonaka et al., 2013, Vanderploeg et al., 2012, Knox et al., 2011, Tsai et al., 2008, Christensen et al., 2007.10.29, Wada et al., 2007)

scb^IIG

(Brown et al., 2000, Martin-Bermudo et al., 1999, Tearle and Nusslein-Volhard, 1987)

Name Synonyms

Secondary FlyBase IDs

References (22)

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