FlyBase Allele Report: Dmel\rhea[1]

General Information

Symbol

Dmel\rhea¹

Species

D. melanogaster

Name

FlyBase ID

FBal0063804

Feature type

allele

Associated gene

Dmel\rhea

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

X ray

Nature of the Allele

Allele class

Mutagen

X ray

(Prout et al., 1997)

Progenitor genotype

Cytology

Description

A frameshift occurs after codon 1139, and the new reading frame terminates after two codons.

(Brown et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3L:8,565,969..8,565,985 [+]

Comment:

Approximate location of small deletion that causes a frameshift after residue 1139. The new reading frame terminates after two codons.

(Brown et al., 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage | somatic clone

(Lin et al., 2013)

decreased cell number | adult stage | somatic clone

(Lin et al., 2013)

lethal - all die during embryonic stage | recessive

(Prout et al., 1997)

some die during embryonic stage | recessive

(Prout et al., 1997)

visible | somatic clone

(Prout, 1997.3.19, Prout et al., 1997)

Phenotype Manifest In

adult posterior midgut epithelium | somatic clone

(Lin et al., 2013)

embryonic/larval somatic muscle cell

(Prout et al., 1997)

wing | somatic clone

(Prout, 1997.3.19, Prout et al., 1997)

Detailed Description

Statement

Reference

Adult-generated rhea¹ homozygous mutant intestinal stem cell clones have reduced maintenance 7 days and 14 days after clone induction compared to control clones; remaining clones contain fewer cells by day 14.

(Lin et al., 2013)

Junction formation between pairs of VL muscles appears normal.

(Deng et al., 2010)

Mutant embryos are indistinguishable from wild type.

(Martin-Bermudo and Brown, 2000)

Homozygous clones in the wing produce discrete, round blisters of variable size. These blisters can be located anywhere on the wing. Wing venation is normal. The somatic musculature completely detaches from the epidermis in homozygous embryos. This phenotype is not seen until very late in embryogenesis, suggesting that the muscles may detach from the epidermis when muscle contractions begin. Attachments between the muscles are maintained.

(Prout et al., 1997)

Homozygous clones in the wing produce a blistered phenotype.

(Prout, 1997.3.19)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

rhea[+]/rhea¹ is an enhancer of visible | recessive phenotype of mys⁸

(Prout et al., 1997)

rhea[+]/rhea¹ is an enhancer of visible | recessive phenotype of mys^b9

(Prout et al., 1997)

Other

Statement

Reference

CAP^49e, rhea¹ has abnormal cell number | embryonic stage phenotype

(Jammrath et al., 2020)

Phenotype Manifest In

Enhancer of

Statement

Reference

rhea[+]/rhea¹ is an enhancer of heart primordium phenotype of sli²

(MacMullin and Jacobs, 2006)

rhea[+]/rhea¹ is an enhancer of wing phenotype of mys⁸

(Prout et al., 1997)

rhea[+]/rhea¹ is an enhancer of wing phenotype of mys^b9

(Prout et al., 1997)

Other

Statement

Reference

CAP^49e, rhea¹ has embryonic cardioblast phenotype

(Jammrath et al., 2020)

rhea¹, scb²/scb[+] has embryonic heart cardioblast phenotype

(Vanderploeg et al., 2012)

rhea¹, sd⁹ has somatic muscle cell phenotype

(Deng et al., 2010)

rhea¹, vg^null has muscle cell of A1-7 ventral longitudinal muscle 1 phenotype

(Deng et al., 2010)

rhea¹, vg^null has muscle attachment site phenotype

(Deng et al., 2010)

Tig^x, rhea¹ has muscle attachment site phenotype

(Martin-Bermudo and Brown, 2000)

Additional Comments

Genetic Interactions

Statement

Reference

At early stages (15), the VL muscles show no significant migration or attachment defects in vg^null; rhea¹ double mutant embryos. However, at later stages (16+) several muscles, most prevalently VL1 can be seen detaching from their normal location. This muscle detachment phenotype appears to be solely due to a lack of vg activity as it can be rescued through expression of vg^{Scer\UAS.T:Ivir\HA1} in muscle cells.

The direction of VL1 muscle migration is unaffected in vg^null; rhea¹ double mutants.

The development of tendon cells is not affected in vg^null; rhea¹ double mutants.

sd⁹; rhea¹ double mutants produce a mild somatic musculature phenotype, but otherwise are indistinguishable from rhea¹ mutants.

The muscle detachment phenotype found in vg^null; rhea¹ double mutant embryos appears to be solely due to a lack of vg activity as it can be rescued through expression of vg^{Scer\UAS.T:Ivir\HA1} in muscle cells.

(Deng et al., 2010)

rhea¹/+; sli²/+ embryos show defects in heart formation.

(MacMullin and Jacobs, 2006)

Tig^x ; rhea¹ double mutant embryos have a severe muscle detachment phenotype compared to either single mutant.

(Martin-Bermudo and Brown, 2000)

Shows a strong genetic interaction with mys⁸ and mys^b9; the frequency of wing blisters and the penetrance of the wings held-out phenotype in mys⁸ or mys^b9 single hemizygotes is increased if they are also heterozygous for rhea¹.

(Prout et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

rhea^13-8

(Levi et al., 2006)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

2296

y¹ w¹; P{white-un1}70C rhea¹ P{neoFRT}80B/TM6B, P{iab-2(1.7)lacZ}6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

rhea¹

(Clarembaux-Badell et al., 2022, Hunter et al., 2020, Jammrath et al., 2020, Sarov et al., 2016, Bharadwaj et al., 2013, Vanderploeg et al., 2012, Knox et al., 2011, Deng et al., 2010, Levi et al., 2006, Levi et al., 2006, MacMullin and Jacobs, 2006)

talin¹

(Lin et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (16)

Report Sections

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