From: Kevin Cook <kercook@XXXX> Date: 6 May 2009 00:42:59 BST To: flybase-cambridgeXXXX, sjchristXXXX, ruacook@XXXX Subject: Isolation and characterization of Df(3R)BSC793 Isolation and characterization of Df(3R)BSC793 Stacey Christensen, Kim Cook and Kevin Cook Bloomington Stock Center Indiana University Df(3R)BSC793 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG1544f06723 and P{XP}d09952. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}CG1544f06723/P{XP}d09952 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC793 from the segment of PBac{WH}CG1544f06723 to the left of its FRT site and the segment of P{XP}d09952 to the right of its FRT site. The breakpoints of Df(3R)BSC793 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 3R:27025841 ;27283862 and the cytological breakpoints predicted from these coordinates are 100B5;100C4. Df(3R)BSC793 failed to complement spn-F1 for female sterility. -- Kevin Cook, Ph.D Bloomington Drosophila Stock Center Department of Biology Indiana University 1001 E. Third St. Bloomington, IN 47405-7005 kercook@XXXX 812-856-1213 (office), 812-855-2577 (fax) http://flystocks.bio.indiana.edu
