FlyBase Allele Report: Dmel\spn-F[1]

General Information

Symbol

Dmel\spn-F¹

Species

D. melanogaster

Name

FlyBase ID

FBal0016043

Feature type

allele

Associated gene

Dmel\spn-F

Associated Insertion(s)

Carried in Construct

Also Known As

spn-F²³⁴

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Abdu et al., 2006)

Mutagen

ethyl methanesulfonate

(Lindsley and Zimm, 1992, Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Nucleotide substitution: ?178?.

(Abdu et al., 2006)

Amino acid replacement: ?59term.

(Abdu et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:31,255,417..31,255,417 [+]

Nucleotide change:

C31255417T

Reported nucleotide change:

?178?

Amino acid change:

Q59term | spn-F-PA; Q59term | spn-F-PB

Reported amino acid change:

?59term

(Abdu et al., 2006)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

spn-F¹/spn-F¹ mutants exhibit abnormal bristle morphology, wherein bristles are short, branched and have a characteristic swollen region.

(Otani et al., 2015)

spn-F¹ mutants exhibit shorter and thicker anterior and posterior scutellar macrochaetae. Approximately 29% of spn-F¹ mutant bristles exhibit wild-type morphology, in which the shaft diameter is the widest at the bristle base and tapered toward the tip. In most of the mutant fly bristles, the widest shaft diameter is not found at the base of the bristle. Approximately 59% of the mutant bristles exhibit an altered growth direction that is not axially biased and 24% terminate in several minitips instead of a single tip. The middle third of the mutant bristles show moderately disorganized ridges that are shallower and more numerous than the wild-type bristle surface, which is characterized by straight ridges and valleys, suggesting that alterations in actin organization occurs in the mutants during the middle phase of bristle elongation.

(Bitan et al., 2010)

Df(3R)BSC793 fails to complement spn-F¹ for female sterility.

(Christensen et al., 2009.5.6)

Females mutant for spn-F¹ produce a ventralised eggshell. A fraction of eggs are fertilised and the embryos that form inside have a variety of pattern abnormalities. Apart from ventralised embryos, some bicaudal embryos are found inside the eggs, ranging from embryos with the typical reduced head skeleton, to rare symmetrical bicaudals with several abdominal segments in mirror image symmetry and Filzkoerper and telson at both ends.

spn-F¹ and spn-F¹/Df(3R)tll-e ventralised eggshells exhibit a variety of phenotypes. The majority of these eggs (approximately 80%) do not have appendages, with smaller percentages exhibiting a single appendage or fusing of the appendages at the base.

(Abdu et al., 2006)

Egg shape affected; in extreme cases dorsal appendages are lacking and eggs have little or no dorsal-ventral polarity; some eggs have one fused dorsal appendage. Low fecundity; eggs often slightly collapsed.

(Lindsley and Zimm, 1992)

Maternal effect mutation similar to spn-A.

(Tearle and Nusslein-Volhard, 1987)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

spn-F¹ has visible | adult stage phenotype, non-enhanceable by Scer\GAL4^sca.PU/IKKε^NIG.2615R

(Otani et al., 2015)

Suppressed by

Statement

Reference

spn-F¹ has visible | adult stage phenotype, suppressible | partially by Scer\GAL4^sca.PU/IKKε^UAS.Tag:MYC

(Otani et al., 2015)

NOT suppressed by

Statement

Reference

spn-F¹ has visible | adult stage phenotype, non-suppressible by Scer\GAL4^sca.PU/IKKε^NIG.2615R

(Otani et al., 2015)

NOT Enhancer of

Statement

Reference

spn-F¹/spn-F¹ is a non-enhancer of visible | adult stage phenotype of IKKε^NIG.2615R, Scer\GAL4^sca.PU

(Otani et al., 2015)

Suppressor of

Statement

Reference

spn-F¹ is a suppressor | partially of abnormal size | oogenesis phenotype of Scer\GAL4^{VP16.mat.αTub67C}, jvl^UAS.mCherry

(Baskar et al., 2019)

NOT Suppressor of

Statement

Reference

spn-F¹/spn-F¹ is a non-suppressor of visible | adult stage phenotype of IKKε^NIG.2615R, Scer\GAL4^sca.PU

(Otani et al., 2015)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

spn-F¹ has mesothoracic bristle phenotype, non-enhanceable by Scer\GAL4^sca.PU/IKKε^NIG.2615R

(Otani et al., 2015)

Suppressed by

Statement

Reference

spn-F¹ has mesothoracic bristle phenotype, suppressible | partially by Scer\GAL4^sca.PU/IKKε^UAS.Tag:MYC

(Otani et al., 2015)

NOT suppressed by

Statement

Reference

spn-F¹ has mesothoracic bristle phenotype, non-suppressible by Scer\GAL4^sca.PU/IKKε^NIG.2615R

(Otani et al., 2015)

NOT Enhancer of

Statement

Reference

spn-F¹/spn-F¹ is a non-enhancer of mesothoracic bristle phenotype of IKKε^NIG.2615R, Scer\GAL4^sca.PU

(Otani et al., 2015)

Suppressor of

Statement

Reference

spn-F¹ is a suppressor | partially of oocyte phenotype of Scer\GAL4^{VP16.mat.αTub67C}, jvl^UAS.mCherry

(Baskar et al., 2019)

NOT Suppressor of

Statement

Reference

spn-F¹/spn-F¹ is a non-suppressor of mesothoracic bristle phenotype of IKKε^NIG.2615R, Scer\GAL4^sca.PU

(Otani et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

spn-F¹/spn-F¹ does not suppress or enhance the abnormal bristle morphology seen in flies expressing IKKε^NIG.2615R under the control of Scer\GAL4^sca.PU, and vice versa.

Expression of IKKε^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^sca.PU partially suppresses the bristle defects seen in spn-F¹/spn-F¹ mutants.

(Otani et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

spn-F¹ is rescued by Scer\GAL4^sca.PU/spn-F^UAS.FL.EGFP

(Otani et al., 2015)

spn-F¹ is rescued by Scer\GAL4^sca.PU/spn-F^{Δ253.UAS.EGFP}

(Otani et al., 2015)

spn-F¹ is rescued by Scer\GAL4^{VP16.nanos.UTR}/spn-F^UAS.cAa

(Abdu et al., 2006)