FlyBase Insertion Report: Dmel\P{PZ}shg[10469]

General Information

Symbol

Dmel\P{PZ}shg¹⁰⁴⁶⁹

Species

D. melanogaster

Name

FlyBase ID

FBti0004086

Feature type

transposable_element_insertion_site

Also Known As

shg-lacZ

Description

Inserted Element

P{PZ}

LINE ID

l(2)10469

Affected gene(s)

Ecol\lacZ, shg

Viability / fertility

Associated allele(s)

Ecol\lacZ^shg-10469, shg¹⁰⁴⁶⁹

Stock availability

None publicly available

Genomic Location

Chromosomal Location

2R ( 57B16 )

Sequence Location

2R:21,056,895..21,056,896 [-]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2R:21,056,895..21,056,896 [-]

(Uemura et al., 1996)

Orientation

Cytological location (computed by FlyBase)

57B16 (inferred by FlyBase from sequence location)

Cytological location (reported)

57B13-57B14 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Genomic location previously reported as 2R:16944401..16944401 .

Sequence Data

Flanking sequence

G00624

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Shindo et al., 2008, Jiang and Crews, 2006, Ikeya and Hayashi, 1999, Jackle and Janning, 1998.8.10, Oda et al., 1998, Vosshall, 1998.8.10, Tanaka-Matakatsu et al., 1996, Meister and Braun, 1995.10)

shg

(Tanaka-Matakatsu et al., 1996)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^shg-10469

Phenotypic Class Phenotype Manifest in Detailed Description

shg¹⁰⁴⁶⁹

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

wandering third instar larval stage

embryonic/larval tracheal section

(Meister and Braun, 1995.10)

imaginal disc

(Meister and Braun, 1995.10)

embryonic/larval brain

(Meister and Braun, 1995.10)

adult stage

third segment of antenna

(Vosshall, 1998.8.10)

lamina

(Vosshall, 1998.8.10)

Additional Information

Statement

Reference

In embryo, expression in mesoderm and mesectoderm not certain.

(Jackle and Janning, 1998.8.10)

In wandering third instar larva, strong expression in the imaginal discs (which discs not specified). Weak expression in the brain and trachea.

(Meister and Braun, 1995.10)

Marker for

Reflects expression of

shg

(Meister and Braun, 1995.10)

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)10469

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (0)

External Crossreferences and Linkouts ( 0 )

Comments

insertion located on reference sequence by FlyBase curator based on the statement in FBrf0086666 that the insertion is 'behind nucleotide 265 in the 5' untranslated region' of the shg cDNA; for flanking sequence see GB:G00624

(Uemura et al., 1996)

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

P{PZ}l(2)10469¹⁰⁴⁶⁹

(FlyBase, 1992-)

P{PZ}shg¹⁰⁴⁶⁹

(Tanaka-Matakatsu et al., 1996, Uemura et al., 1996, FlyBase, 1992-)

P{ry^+t7.2=PZ}l(2)10469¹⁰⁴⁶⁹

(FlyBase, 1992-)

P{ry^+t7.2=PZ}shg¹⁰⁴⁶⁹

(FlyBase, 1992-)

shg-lacZ

(Shindo et al., 2008, Jiang and Crews, 2006, Ikeya and Hayashi, 1999)

Name Synonyms

Secondary FlyBase IDs

FBti0001349
FBti0000435

References (19)

Report Sections

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