FB2024_03 , released June 25, 2024
Allele: Dmel\shg10469
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General Information
Symbol
Dmel\shg10469
Species
D. melanogaster
Name
FlyBase ID
FBal0008151
Feature type
allele
Associated gene
Dmel\shg
Associated Insertion(s)
P{PZ}shg10469
Carried in Construct
Also Known As
shg-lacZ
Key Links
Genomic Maps

Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Uemura et al., 1996)
Mutagen
P-element activity
Progenitor genotype
Associated Insertion(s)
P{PZ}shg10469
Cytology
Description

P{PZ} is inserted behind nucleotide 265 in the 5' untranslated region of the shg transcription unit, such that the Ecol\lacZ coding region is in the opposite orientation to the shg coding region.

(Uemura et al., 1996)
Allele components
Component
Use(s)
Inserted element
P{PZ}
Encoded product / tool
lacZ
Tagged with
Tag:NLS(P)
Mutations Mapped to the Genome
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      7-day old shgR69/+ germaria have normal numbers of germline stem cells (GSCs), but at 63-days old, these germaria have significantly lower GSC numbers and contain significantly fewer cysts than controls, while cap cells are maintained at normal numbers.

      (Pan et al., 2007)

      Only 8.9% of marked shg10469 somatic stem cells (SSCs) observed during the first week after clone induction in the adult ovary are maintained for 2 more weeks, suggesting that the mutant SSCs are severely destabilised (46% of marked wild-type SSCs observed during the first week after clone induction are maintained for 2 more weeks). Mutant shg10469 oocytes are always localised to the posterior end of the egg chamber.

      (Song and Xie, 2002)

      shg10469 has no dramatic effect on germline stem cell (GSC) maintenance in the ovary; 60% of mutant GSCs observed during the first week after clone induction are maintained for two more weeks (compared to 70% for controls).

      (Song et al., 2002)

      Adult escapers show a patterning defect along the anterior wing margin. Mutants show gross disorganization of epithelial tissues in the head, ventral epidermis, Malpighian tubules and tracheal ducts. Malpighian tubule tip cells are present and differentiate into neurons, as in wild type. Tracheal tip cells are also present.

      (Uemura et al., 1996)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT Suppressor of
      Statement
      Reference

      shg10469 is a non-suppressor of female germline stem cell | germline clone phenotype of bgcn20915

      (Jin et al., 2008)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Germline stem cell (GSC) clones carrying both bgcn20915 and shg10469 outcompete wild type GSCs similarly to GSCs mutant for bgcn20915 alone.

      (Jin et al., 2008)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      A. Spradling.

      Comments
      Comments

      Complements: l(2)0062900629. Complements: l(2)0305003050. Complements: insc05475. Complements: l(2)0720607206. Complements: l(2)0780607806. Complements: insck12405. Complements: Xbp1k13803.

      (BDGP Project Members, 1994-1999)

      Transposase induced excision is accompanied by reversion of the mutant phenotype.

      (Uemura et al., 1996)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      P2370
      (Tanaka-Matakatsu et al., 1996)
      l(2)10469
      (Spradling et al., 1999)
      l(2)1046910469
      shg10469
      (Inaba et al., 2015, Jin et al., 2008, Pan et al., 2007)
      shgP10469
      (Uemura et al., 1996)
      Name Synonyms
      Secondary FlyBase IDs
        References (11)