FB2024_03 , released June 25, 2024
Insertion: Dmel\P{PZ}sli05248
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General Information
Symbol
Dmel\P{PZ}sli05248
Species
D. melanogaster
Name
FlyBase ID
FBti0006990
Feature type
transposable_element_insertion_site
Also Known As
slit-lacZ
Description
Inserted Element
P{PZ}
LINE ID

l(2)05248

Affected gene(s)
bdg, Ecol\lacZ, sli
Viability / fertility
Associated allele(s)
Ecol\lacZsli-05248, sli05248
Stock availability
1 publicly available
Genomic Location
Chromosomal Location
2R ( 52D2 )
Sequence Location
2R:15,922,111..15,922,111 [+]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_P{PZ}.BDGP
Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:15,922,111..15,922,111 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)
52D2 (inferred by FlyBase from sequence location)
Cytological location (reported)
52D1-52D2 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
AQ025633
(FlyBase, 1992-)
Inserted Element
Construct
P{PZ}
Size (Kb)

14.545

Component Allele(s)
l(3)87Df7.2
ry+t7.2
Molecular map
Affected Gene(s)
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
adult medulla neuropil associated glial cell
(Tayler et al., 2004)
medulla anlage
(Tayler et al., 2004)
adult stage
region of integument | restricted | ventral
(Fabre et al., 2010)
region of integument | dorsal | restricted of adult abdominal segment posterior compartment
(Fabre et al., 2010)
Additional Information
Statement
Reference

Dorsally, sli reporter expression is observed in most of the abdominal segment posterior compartments. Expression is stronger laterally and towards the posterior boundary of the compartment. Ventrally, sli reporter expression is observed in non-sternite regions of integument in lines following the anterior-posterior axis. Expression extends across the midline in regions approximately coextensive with the abdominal segment posterior compartments on the dorsal side.

(Fabre et al., 2010)

EcollacZsli-05248 is expressed in medulla glia and cells of the medulla cortex in the developing larval optic lobe.

(Tayler et al., 2004)
 
Marker for
Reflects expression of
sli
(Tayler et al., 2004)
Reporter construct used in assay
Data on Genetic Line
Line ID

l(2)05248

(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Comments
Comments

Location 2R:11436974-11436975 confirmed by FlyBase alignment of dbGSS accession AQ025633 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.

(FlyBase, 2005)

The P{PZ}l(2)0524805248 insertion maps 1070bp downstream of the 3\' end of the CT21678 transcript of CG8291.

(Misra, 2000.8.25)
Synonyms and Secondary IDs (6)
Reported As
Symbol Synonym
PPZslit05248
(Weyers et al., 2011)
P{PZ}l(2)0524805248
(FlyBase, 2005, BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{PZ}sli05248
(FlyBase, 1992-)
sli.lacZ
(Fabre et al., 2010)
slit-LacZ
(Anllo and DiNardo, 2022)
slit-lacZ
(Plazaola-Sasieta et al., 2019)
Name Synonyms
Secondary FlyBase IDs
    References (13)