FB2024_03 , released June 25, 2024
Insertion: Dmel\P{GawB}NP3084
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General Information
Symbol
Dmel\P{GawB}NP3084
Species
D. melanogaster
Name
FlyBase ID
FBti0035148
Feature type
transposable_element_insertion_site
Also Known As
NP3084-GAL4, NP3084
Description
Inserted Element
P{GawB}
LINE ID
Affected gene(s)
Scer\GAL4
Viability / fertility
viable
Associated allele(s)
Scer\GAL4NP3084
Stock availability
1 publicly available
Genomic Location
Chromosomal Location
2L ( 31F4 )
Sequence Location
2L:10,506,787..10,506,787 [-]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_P{GawB}.NP
Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

(Hayashi et al., 2002)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
2L:10,506,787..10,506,787 [-]
(FlyBase, 2005)
Orientation
Cytological location (computed by FlyBase)
31F4 (inferred by FlyBase from sequence location)
Cytological location (reported)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
AG217195
(FlyBase, 1992-)
Inserted Element
Construct
P{GawB}
Size (Kb)

11.279

Component Allele(s)
Scer\GAL4wB
w+mW.hs
Molecular map
Affected Gene(s)
Insertion may affect gene
Scer\GAL4
(Lang et al., 2012, Ha et al., 2009, Ha et al., 2009, Wang et al., 2009, Croker et al., 2007)
Variant Molecular Consequences
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
larval stage
embryonic/larval midgut
(Yin et al., 2017, Wang et al., 2009)
embryonic/larval salivary gland
(Wang et al., 2009)
gastric caecum
(Wang et al., 2009)
wandering third instar larval stage
embryonic/larval midgut
(Tang and Zhou, 2013)
embryonic/larval tracheal section
(Tang and Zhou, 2013)
embryonic/larval nervous system | restricted
(Tang and Zhou, 2013)
adult stage
adult gut
(Croker et al., 2007)
adult midgut
(Bonnay et al., 2013)
enterocyte
(Lee et al., 2016)
Additional Information
Statement
Reference

Drives expression in the larval and adult midgut, and in the larval gastric caecum.

(FlyBase Curators, 2017)

NP3084 is expressed strongly in the whole larval midgut and mildly in other places including a small subset of neural cells and trachea cells.

(Tang and Zhou, 2013)
 
Marker for
Reflects expression of
Reporter construct used in assay
SurRNAi.UAS.1-2
(Croker et al., 2007)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 1 )
Crossreferences
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
Comments
Comments

Location 2L:10506786-10506787 refined by FlyBase alignment of dbGSS accession AG217195 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.

(FlyBase, 2005)
Synonyms and Secondary IDs (15)
References (39)