FlyBase Insertion Report: Dmel\P{GawB}hfw[NP7175]

General Information

Symbol

Dmel\P{GawB}hfw^NP7175

Species

D. melanogaster

Name

FlyBase ID

FBti0058526

Feature type

transposable_element_insertion_site

Also Known As

NP7175-GAL4, NP7175

Description

Inserted Element

P{GawB}

LINE ID

Affected gene(s)

hfw, Scer\GAL4

Viability / fertility

Associated allele(s)

hfw^NP7175, Scer\GAL4^NP7175

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

X ( 2B5 )

Sequence Location

X:1,669,711..1,669,981

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{GawB}.NP

Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w^+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

(Hayashi et al., 2002)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

X:1,669,711..1,669,981

(FlyBase, 2005)

Orientation

Cytological location (computed by FlyBase)

2B5 (inferred by FlyBase from sequence location)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AG216532

(FlyBase, 1992-)

Inserted Element

Construct

P{GawB}

Size (Kb)

11.279

Component Allele(s)

Scer\GAL4^wB

w^+mW.hs

Molecular map

Affected Gene(s)

Insertion may affect gene

hfw

(FlyBase Curators, 2008-)

Scer\GAL4

(Pauls et al., 2010, Aso et al., 2009, Tanaka et al., 2008, Tanaka et al., 2004)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

hfw^NP7175

Phenotypic Class Phenotype Manifest in Detailed Description

Scer\GAL4^NP7175

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

inner layer of the larval mushroom body

(Pauls et al., 2010, Pauls et al., 2010)

outer layer of the larval mushroom body

(Pauls et al., 2010, Pauls et al., 2010)

core layer of the larval mushroom body

(Pauls et al., 2010, Pauls et al., 2010)

adult stage

adult antennal lobe projection neuron | subset

(Tanaka et al., 2004)

adult mushroom body alpha-lobe | restricted

Comment: core of mushroom body alpha-lobe

(Tanaka et al., 2004)

adult mushroom body beta-lobe | restricted

Comment: core of mushroom body beta-lobe

(Tanaka et al., 2004)

calyx of adult mushroom body

(Tanaka et al., 2008)

mushroom body beta lobe core layer | subset

(Tanaka et al., 2008)

mushroom body alpha lobe core layer | subset

(Tanaka et al., 2008)

adult mushroom body | restricted

(Tanaka et al., 2004)

alpha/beta Kenyon cell

(Pauls et al., 2010)

alpha/beta core Kenyon cell

(Flybrain Neuron Database et al., 2018.6.28, Perisse et al., 2013)

Kenyon cell

(Flybrain Neuron Database et al., 2018.6.28)

adult mushroom body alpha-lobe

(Pauls et al., 2010)

adult mushroom body beta-lobe

(Pauls et al., 2010)

adult stage | female

mushroom body beta lobe core layer

(Aso et al., 2009)

mushroom body alpha lobe core layer

(Aso et al., 2009)

medulla

(Aso et al., 2009)

adult tritocerebrum

(Aso et al., 2009)

adult gnathal ganglion | faint

(Aso et al., 2009)

glial cell of adult brain | faint

(Aso et al., 2009)

adult stage | male

mushroom body beta lobe core layer | intense

(Aso et al., 2009)

mushroom body alpha lobe core layer | intense

(Aso et al., 2009)

medulla | intense

(Aso et al., 2009)

adult tritocerebrum

(Aso et al., 2009)

adult gnathal ganglion | faint

(Aso et al., 2009)

glial cell of adult brain

(Aso et al., 2009)

Additional Information

Statement

Reference

Expression of ScerGAL4^NP7175 is found within the inner regions of the alpha and beta core stratum and not the outer core.

(Tanaka et al., 2008)

Expression is observed in 46-70 projection neurons in the cores of the α and β lobes of the mushroom body.

(Tanaka et al., 2004)

Marker for

Reflects expression of

Reporter construct used in assay

Mmus\Cd8a^UAS.cLa.EGFP

(Pauls et al., 2010, Aso et al., 2009, Tanaka et al., 2008)

Avic\GFP^S65T.UAS

(Tanaka et al., 2008)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Kyoto

114120

P{GawB}hfw^NP7175 w^* / FM7c

External Crossreferences and Linkouts ( 1 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

AG216532

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

NP 7175

(Pauls et al., 2010, Pauls et al., 2010)

NP7175

(Flybrain Neuron Database et al., 2018.6.28, Perisse et al., 2013, Huang et al., 2012, Pauls et al., 2010, Tanaka et al., 2008, Tanaka et al., 2004)

NP7175-5-1

(FlyBase, 1992-)

NP7175-GAL4

(Haussmann et al., 2022, Vrontou et al., 2021, Wu et al., 2020, Groschner et al., 2018, Beshel et al., 2017, Tong et al., 2016, DasGupta et al., 2014, Aso et al., 2009)

NP7175-Gal4

(Kelly et al., 2016, Shimizu et al., 2011, Shin and Diantonio, 2011)

NP7175-gal4

(Jia et al., 2019, Abe et al., 2014)

P{GawB}NP7175

(Tanaka et al., 2004, FlyBase, 1992-)

P{GawB}NP7175-5-1

(FlyBase, 1992-)

P{GawB}hfw^NP7175

Name Synonyms

Secondary FlyBase IDs

FBti0037278

References (23)

Report Sections

Open Close