FlyBase Insertion Report: Dmel\P{GawB}trol[NP5103]

General Information

Symbol

Dmel\P{GawB}trol^NP5103

Species

D. melanogaster

Name

FlyBase ID

FBti0058532

Feature type

transposable_element_insertion_site

Also Known As

NP5103, NP5103-GAL4

Description

Inserted Element

P{GawB}

LINE ID

Affected gene(s)

Scer\GAL4, trol

Viability / fertility

Associated allele(s)

Scer\GAL4^NP5103, trol^NP5103

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

X ( 3A4 )

Sequence Location

X:2,517,400..2,517,651

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{GawB}.NP

Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w^+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

(Hayashi et al., 2002)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

X:2,517,400..2,517,651

(FlyBase, 2005)

Orientation

Cytological location (computed by FlyBase)

3A4 (inferred by FlyBase from sequence location)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AG217853

(FlyBase, 1992-)

Inserted Element

Construct

P{GawB}

Size (Kb)

11.279

Component Allele(s)

Scer\GAL4^wB

w^+mW.hs

Molecular map

Affected Gene(s)

Insertion may affect gene

Scer\GAL4

(Kazama et al., 2011, Sweeney et al., 2011, Pauls et al., 2010, Donlea et al., 2009, Sakurai et al., 2009, Tanaka et al., 2008, Komiyama et al., 2007, Olsen et al., 2007, Tanaka et al., 2004)

trol

(FlyBase Curators, 2008-)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Scer\GAL4^NP5103

Phenotypic Class Phenotype Manifest in Detailed Description

trol^NP5103

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult antennal lobe projection neuron | subset

(Tanaka et al., 2004)

antennal lobe glomerulus VM2

(Tanaka et al., 2004)

adult lateral antennal lobe tract

(Tanaka et al., 2008)

calyx of adult mushroom body

(Tanaka et al., 2008)

adult antennal lobe projection neuron VM2 adPN

(Flybrain Neuron Database et al., 2018.6.28, Tanaka et al., 2012)

lateral antennal lobe tract projection neuron | subset

(Tanaka et al., 2012)

lateral antennal lobe tract projection neuron

(Flybrain Neuron Database et al., 2018.6.28)

Additional Information

Statement

Reference

ScerGAL4^NP5103 drives expression in three uniglomerular antennal lobe projection neurons. Two cell bodies are positioned in the anterior-dorsal part of the antennal lobe and they project to antennal lobe glomerulus VM2 only. One cell body is positioned in the lateral part and innervates glomerulus VP1.

(Tanaka et al., 2012)

Expression is observed in 2 projection neurons in antennal glomerulus VM2.

(Tanaka et al., 2004)

Marker for

adult antennal lobe projection neuron VM2 adPN

(Tanaka et al., 2004)

adult lateral antennal lobe tract

(Tanaka et al., 2008)

Reflects expression of

Reporter construct used in assay

Mmus\Cd8a^UAS.cLa.EGFP

(Tanaka et al., 2012, Tanaka et al., 2008)

Avic\GFP^S65T.UAS

(Tanaka et al., 2008)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Kyoto

113584

P{GawB}trol^NP5103 w^* / FM7c

External Crossreferences and Linkouts ( 1 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

AG217853

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

NP5103

(Flybrain Neuron Database et al., 2018.6.28, Kidd et al., 2015, Tanaka et al., 2012, Pauls et al., 2010, Donlea et al., 2009, Sakurai et al., 2009, Tanaka et al., 2008, Komiyama et al., 2007, Tanaka et al., 2004)

NP5103-5-1

(FlyBase, 1992-)

NP5103-GAL4

(Xie et al., 2019, Li et al., 2017, Sweeney et al., 2011)

NP5103-Gal4

(Sekine et al., 2013, Kazama et al., 2011, Olsen et al., 2007)

P{GawB}NP5103

(Tanaka et al., 2004, FlyBase, 1992-)

P{GawB}NP5103-5-1

(FlyBase, 1992-)

P{GawB}trol^NP5103

trol-GAL4

(Li et al., 2017)

Name Synonyms

Secondary FlyBase IDs

FBti0036362

References (18)

Report Sections

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